TECHNOLOGICAL PARAMETERS OF PRODUCTION OF LIPOSOMES WITH OXALIPLATIN

Authors

  • O. V. Stadnichenko National University of Pharmacy
  • Yu. M. Krasnopolsky National Technique University “Kharkiv Polytechnic Institute”
  • T. G. Yarnykh National University of Pharmacy

DOI:

https://doi.org/10.11603/2312-0967.2018.1.8612

Keywords:

oxaliplatin, liposomes, pharmaceutical development, technology, chromatography, lyophilization.

Abstract

The development of medicines of a nanoscale diapason opens a new trend in the modern pharmaceutical industry. Reducing the toxicity of known cytotoxic agents is one of the advantages of the liposomal biotechnology platform. The aim of the work is to offer scientifically grounded technology of production of liposomal form of oxaliplatin based on the experiment. To analyze intermediate products, define control points.

Materials and Methods. Egg phosphatidylcholine produced byLipoid,Germany was used to make liposomes. Dipalmitoylphosphatidylglycerin, cholesterol, solvents used by the companySigma-Aldrich,USA. The lipid film was obtained on a rotary evaporator Buchi 210. For homogenization, an extrusion method at high pressure was used. The lyophilization process was carried out on Quarco equipment (PRC).

Results and Discussion. The experimental industrial technology of obtaining the liposomal form of oxaliplatin was developed. The development of a full-fledged technology requires the study of colloidal properties of a heterogeneous nano-system, especially at the rehydration stage, lyophilization parameters, validation of control methods for the encapsulation of oxaliplatin into liposomes, validation of quantitative determination and determination of impurities.

Conclusions. A new, original technology and control points for obtaining liposomal form of oxaliplatin are proposed. The technology has been tested on batches of preparations, used for preclinical studies.

Author Biographies

O. V. Stadnichenko, National University of Pharmacy

Candidate of Pharmaceutical Sciences, Applicant of a Scientific Degree, Drugs Technology Department, National University of Pharmacy

Yu. M. Krasnopolsky, National Technique University “Kharkiv Polytechnic Institute”

Doctor of Pharmaceutical Sciences, Professor, National Technique University “Kharkiv Polytechnic Institute”

T. G. Yarnykh, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Honored Worker of Science and Technique of Ukraine, Laureate of the State Prize of Ukraine in Science and Technique, Head of Drugs Technology Department, National University of Pharmacy

References

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Published

2018-03-24

How to Cite

Stadnichenko, O. V., Krasnopolsky, Y. M., & Yarnykh, T. G. (2018). TECHNOLOGICAL PARAMETERS OF PRODUCTION OF LIPOSOMES WITH OXALIPLATIN. Pharmaceutical Review Farmacevtičnij časopis, (1), 57–62. https://doi.org/10.11603/2312-0967.2018.1.8612

Issue

No. 1 (2018)

Section

Pharmaceutical technology, biopharmacy, homeopathy, biotechnology

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