SELECTION OF QUANTITATIVE COMPOSITION FOR THE DEVELOPMENT OF THE VETERINARY PREPARATION FOR INTRAMAMMARY INTRODUCTION
DOI:
https://doi.org/10.11603/2312-0967.2017.4.8336Keywords:
veterinary drug, intramammary administration, stability, silver citrate.Abstract
The aim of the work. Development of qualitative composition and selection of the optimal ratio of the components of the combined veterinary drug for intramammary administration.
Materials and Methods. The study object was a series of preparations of the antimicrobial action "Argocide", which containing silver citrate, D-panthenol and L-arginine and is intended for the treatment and prevention of subclinical mastitis in cattle. Physico-chemical stability parameters of the preparation were studied at a temperature of 25 ± 2 ° C for long-term tests and at a temperature of 40 ± 2 ° C for accelerated tests; defined the following parameters in accordance with the requirements of the State Pharmacopoeia of Ukraine: the quantitative content of active substances, the appearance of the solution, transparency, pH.
Results and Discussion. As a result of the study of stability samples of the veterinary drug during storage with the following proportions of active substances: silver citrate – 0.01 g, L-arginine – 0.52 g, dexpanthenol – 0.50 g, it was found that, under long-term storage conditions, physical and chemical properties for 6 months did not change. The results of the accelerated tests revealed the instability of samples of the veterinary drug at earlier times, which may be a consequence of the destruction of silver citrate in the test samples. A research work was carried out to study the stability of the selected combination of active substances after changing its concentration in solution. The study was carried out by analyzing the results of observations of the stability of samples obtained by two methods. According to the first method, a veterinary dosage form was obtained, which is two sterile solutions – A and B, which are mixed before administration in a single syringe tube for intramammary use. The second method was different in that the solution was prepared immediately with the same concentrations of all components as in a mixed solution of A + B, with the selected ratio of active substances being the same.
Conclusions. The optimal ratio of the components of a new combination intramammary preparation of antimicrobial action in the form of a solution in ampoules containing silver citrate 0.005 g, D-panthenol 0.260 g, and L-arginine 0.250 g for the treatment and prophylaxis of subclinical mastitis of cattle under the code name "Argocid", which is stable during storage for 12 months.
References
Busenko OT, Skotsyk VYe, Matsenko MI. Technology of products animal husbandry [Технологія виробництва продукції тваринництва] Kyiv: Ahroosvita; 2013. Ukrainian.
Weese JS, Giguère S, Guardabassi L, Morley PS, Papich M, Ricciuto DR, Sykes JE. ACVIM consensus statement on therapeutic antimicrobial use in animals and antimicrobial resistance. J Vet Intern Med. 2015;29(2): 487-98. Available from: http://dx.doi.org/ DOI:10.1111/jvim.12562.
Panchenko AA, Zagorulko MP, Voytenko LG. A new approach to the treatment of mastitis in cows. In: Youth Scientific Forum: Natural and Medical Sciences. 2014;10(16): 18-28. Available from: http://www.nauchforum.ru/archive/MNF_nature/10(16).pdf. Russian. Abdessemed D, Avdeenko AV. [Diagnosis and therapy of subclinical mastitis in lactating cows]. Vest Sarat Gos Un-ta im NI Vavilova. 2014;3: 3-6. Russian.
Zimnikov VI, Klimov NT, Vostroilova GA. [Pharmacological and toxicological characteristics of the new anti-mastitis drug Masticef]. Zootechn.2010;2: 28-9. Russian.
Yablonskyi VA, Khomin SP, Kalynovskyi HM, Kharuha HH, Kharenko MI, Zaviriukha VI, Liubetskyi VY. Veterinary obstetrics, gynecology and biotechnology of reproduction of animals with the basics of andrology. [Ветеринарне акушерство, гінекологія та біотехнологія відтворення тварин з основами андрології]. Vinnytsia: Nova knyha; 2006. Ukrainian.
Bezuhlyi PO, Ukrainets IV, Taran SH. Pharmaceutical chemistry. [Фармацевтична хімія] Kharkiv: National University of Pharmacy; 2002. Ukrainian.
State Pharmacopoeia of Ukraine: in 3 vol. / State En¬terprise "Ukrainian Research Center expert pharmacopoe¬ia quality medicines." – 2nd ed. – Kharkiv State Enterprise "Ukrainian Research Center expert pharmacopoeia drug quality," 2015;1: 1128. Ukrainian.
Heorhiievskyi V, Liapunov M, Bezuhla O. Guidelines 42-3.3: 2004. Guidelines for quality: Medicines. Stability test. Kyiv: MOZ Ukrainy, 2004. Ukrainian.
Metodychni rekomendatsii shchodo osnovnykh pravyl nalezhnoi praktyky vyrobnytstva ta kontroliu yakosti veterynarnykh preparativ [Methodological recommendations on the basic rules of good manufacturing practice and quality control of veterinary drugs]. Available at: http://www.vet.gov.ua/node/1819.
Commission Directive 91/412/EEC of 23 July 1991 laying down the principles and guidelines of good manufacturing practice for veterinary medicinal products. J of the Eur Com L 228. 1991; 70-3.
Electronic Medicines Compendium (eMC) Available at: www.resourceclinical.com/parenteral-drug-ther.
Yаtusevich AI, Tolkach NG, Yаtusevich IA, Pankovets EA. Medical preparation in veterinary medicine. [Лекарственные средства в ветеринарной медицине] Moscow: Tekhnoperspektiva; 2006. Russian.
Georgievskiy VP, Konev FA. Technology and standardization of medicines. [Технология и стандартизация лекарств] Kharkov: Rireg 2000. Russian.
Budnikova TM., Hulpa VS, Shmatenko OP, Almakayeva LG. [Production of ampoules for medical purposes]. Pharm Journ. 2004;4: 34-40. Ukrainian.
Krasnyuk II, Mikhaylova GV, Valevko SA. Pharmaceutical technology: Technology of dosage forms. [Фармацевтическая технология: Технология лекарственных форм] Moscow: Academia; 2004. Russian.
Guidelines 42-4.2: 2011. Guidelines for quality: Medicines. Quality Risk Management (ICH Q9). Kyiv: MOZ Ukrainy, 2011. Ukrainian.
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