INFLUENCE OF THE OINTMENT, WHICH CONTAINS THE DRY EXTRACT OF LICORICE RHIZOME, ON THE PHAGOCYTIC ACTIVITY OF BLOOD NEUTROPHIL GRANULOCYTES IN GUINEA PIGS WITH EXPERIMENTAL DERMATITIS
DOI:
https://doi.org/10.11603/2312-0967.2016.4.7120Keywords:
extract of licorice rhizome, immunotropic activity, allergic contact dermatitis.Abstract
Introduction
Allergic contact dermatitis (ACD) - one of the most widespread diseases in dermatological practice [5, 15], which is growing rapidly in all age groups in Ukraine [14]. To solve problem of global prevalence and intensive increase of ACD is need to improve medical provision of the population ofUkraineby drugs for effective treatment of allergic skin diseases. Drugs with licorice (Glycyrrhiza glabra/uralensis) [9] are promising for use in topical medication of ACD as they have a wide range of biological activity and exhibit anti-inflammatory, anti-allergic, immunomodulating activity [1, 2]. The aim of this study was to investigate the influence of the ointment "Allergolik", which contains the dry extract of licorice rhizome, on the nonspecific immunity of animals on the experimental model of ACD caused by 2,4-dinitrochlorobenzene.
Materials and methods
In the experiment were used 24 Guinea pigs 370 –410 gweight. Experimental animals were divided into 4 groups (6 animals in each group): I group– intact control, II group – positive control – untreated animals with ACD, III group – experimental animals with ACD wich were treated with the ointment "Allergolik", IV group – reference group – animals with ACD were treated with reference drug – ointment "Fladeks."
The ointment codenamed "Allergolik" was developed at the Department of Drug Technology of National University of Pharmacy. As a reference drug was used ointment "Fladeks" (LLC "Pharmaceutical company "Zdorovie",Kharkov,Ukraine).
Modeling of ACD was performed by the method Zalkan P. M., Iyevleva E. A. [4]. Treatment of the animals began from the first day of sensitization.
To assess the activity of nonspecific immunity, phagocytic activity of neutrophil granulocytes was determined by phagocytic index (PhI – the number of active phagocytes), phagocytic number (PhN – the average number of yeast cells in one neutrophil) and phagocytosis completion index (PhCI – ability of phagocytes to digest yeast cells) [7] on the 7-th day after application of provoking dose of allergen (27-th day of the experiment).
Results and discussion
On the 7-th day after application of provoking dose of allergen a significant increase of active neutrophils number and absorbing properties of neutrophils were observed in groups II - IV in 1,2-1,4 times compared with the intact control group (I). These data reflect existence of natural immune defense, aimed at localization of allergen and its subsequent removal from the body. At the same time, in the group of animals treated with the drug "Alerholik" (group III) PhI was significantly lower compared with the positive control group (II), which correspond with a reduction of recovery terms and skin inflammation decrease [6]. In all investigated groups digesting ability of phagocytes was preserved (PhCI > 1), characterizing an adequate immune response on the backdrop of ACD. However, PhCI in the positive control group (II) was significantly lower, but in groups III, IV, who were in preventive and therapeutic treatments, the same with intact control group (I).
Thus, on the experimental model of ACD in Guinea pigs, it was shown that the ointment "Alerholik" with the dry extract of licorice rhizome had a strong immunotropic activity concerning to functional activity of neutrophil granulocytes, wich according to the results of [12], has positive impact on the rapid relief of aggravation of skin allergies, shortening of cure period, prevents recurrence and prolongs disease remission.
Conclusions
1. On the experimental model of ACD in Guinea pigs ointment "Alerholik" had a strong immunotropic activity, which manifested in significant decrease of phagocytic activity of neutrophil granulocytes relatively to positive control group, which was not observed in the group of animals treated with a reference drug – "Fladeks".
2. The results were obtained indicate the availability of ointment "Alergolik" further study for the purpose of its use in the treatment of allergic skin diseases.
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