STUDY OF ORGANIC ACIDS IN THE HERB OF TWO ANEMONE L. SPECIES
DOI:
https://doi.org/10.11603/2312-0967.2025.2.15280Keywords:
Ranunculaceae, Anemone, herb, organic acids, gas chromatography-mass-spectrometry, ascorbic acid, phytochemical studies, qualitative composition, quantitative composition, medicinal plantsAbstract
The aim of the work. Identification and quantitative determination of organic acids in the herb of two Anemone species.
Materials and Methods. The research objects were the herbs of Anemone (A.) nemorosa and Anemone ranunculoides harvested in Ivano-Frankivsk region in 2023. Organic acids were identified using the GC/MS method. Chromatographic separation was performed on a GC/MS system Agilent 6890N/5973inert (Agilent Technologies, USA). Quantitative determination of free organic acids and ascorbic acid was carried out by titrimetric methods.
Results and Discussion. The GC/MS method allowed for the identification and quantification of 7 organic acids in the herb of A nemorosa L. and 8 – in A. ranunculoides L., respectively.
Using the titrimetric method, the highest content of organic acids was found in the raw material of A. nemorosa collected near the Pavlivka village, Ivano-Frankivsk region – 0.91 ± 0.01 %. The maximum ascorbic acid content was determined in the herb of A. nemorosa collected near the Ozeryany village, Ternopil region (0.11 ± 0.006 %). In the herb of A. ranunculoides, the content of free organic acids and ascorbic acid amounted to 0.90 ± 0,01 % and 0.07 ± 0,009 %, respectively.
Conclusions. The component composition of organic acids was determined in the samples of Anemone nemorosa and Anemone ranunculoides. The common acids for both species were: oxalic, malonic, succinic, malic, glutaric, and citric acids. In both Anemone nemorosa L. and Anemone ranunculoides L., malic acid was predominant (693.07 and 385.46 µg/g, respectively).
Titrimetric determination of the total content of organic acids and ascorbic acid in the aerial parts indicates variability of these parameters depending on the location of raw material collection.
The results obtained provide a foundation for further phytochemical and pharmacological studies.
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