ASSESSMENT OF GLECHOMA HEDERACEA L. EXTRACTS INFLUENCE ON ESCHERICHIA COLI, BACILLUS SUBTILIS AND CANDIDA PARAPSILOSIS
DOI:
https://doi.org/10.11603/2312-0967.2024.2.14759Keywords:
Glechoma hederacea, total phenols content, total hydroxycinnamic acids content, antimicrobial activityAbstract
The aim of the work. To investigate the interdependence of the total phenols content (TPC), the total hydroxycinnamic acids content (THC) in the extracts of G. hederacea grass and their antimicrobial activity.
Materials and Methods. Extraction under reflux conditions and maceration for G. hederacea extracts preparation; spectrophotometry for TPC and THC determination; method of diffusion in agar for antimicrobial investigation; correlational analysis, namely, Pearson's linear pairwise correlation.
Results and Discussion. The content of polyphenolic compounds in the studied aquatic ethanol (AE) extracts varied between 2.890 and 17.076 mg∙g-1DW in gallic acid equivalent. Extracts with AE of various concentrations exhibited a diameter of the growth zone retardation (ZR) of 8.67-15.67 mm, 8.67-15.00 mm, and 17.00-21.00 mm with Escherichia coli, Bacillus subtilis and Candida parapsilosis, respectively. A close relationship was established between the zones of inhibition and the biologically active substances (BAS) of the extract with 95% AE of G. hederacea (correlation matrix 1). A negative correlation was noted between the ZR of C. parapsilosis and TPC (in terms of gallic acid) with r≥-0.806 (р<0.053), THC (in terms of chlorogenic acid) r≥-0.747 (р<0.088) and a positive correlation with THC (in terms of caffeic acid) r≥+0.856 (р<0.03). Correlations between other ZR of microorganisms and the content of studied BASs were not found.
Conclusions. The phytochemical screening of hydroalcoholic extracts of G. hederacea showed the presence of pharmacologically active substances such as polyphenols, in particular hydroxycinnamic acids (НСA) in significant quantities. It is obvious that the species G.hederacea has excellent antimicrobial properties against E. coli, B. subtilis and C. parapsilosis, and can also be used as a potential source of compounds with antibacterial activity, even though close relationships were established with a positive correlation between the diameter of growth ZR of C. parapsilosis with THC (in equivalent of caffeic acid) of the extract prepared with 95% AE only.
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