PERIPHERAL BLOOD LYMPHOCYTES PHENOTYPIC COMPOSITION IN PATIENTS WITH CERVICAL PAPILOMAVIRUS INFECTIONS, WHICH IgG ANTIBODY TO HERPES SIMPLE VIRUSES WERE DETERMINED

Abstract

The aim of the work – to determine the phenotypic composition of peripheral blood lymphocytes in patients with cervical papillomavirus infection (PVI), whose blood serum showed IgG antibodies to herpes simplex viruses with (HSV)-I and/or (HSV)-2.

Materials and methods. 43 women (average age 26.5 years old) with human papillomavirus (HPV)-induced cervical papillomavirus infection diseases diagnosed by molecular-biological, colposcop, and cytological methods of examination were inspected. Serum screening for anti-HSV-1 and anti-HSV-2 IgG as well as examination of anti-HSV-2 IgG avidity index were examined by solid-phase enzyme-linked immunosorbent assay. The phenotypic composition of peripheral blood lymphocytes were examined by the flow cytofluorometry method using monoclonal antibodies (Beckman Coulter, USA) to CD3⁺, CD4⁺, CD8⁺, CD3⁺/HLA-DR⁺, CD3⁺/CD16⁺/€D56⁺, CD4⁺45RA⁺ CD16⁺/CD56⁺/CD8⁺ and CD16⁺/CD56⁺ antigens. The count of cells and data analysis were performed by cytofluormeter FC-500 (Beckman Coulter, США). The count of cells and analysis of the results were performed by cytofluormeter i. IgG antibodies to HSV-1, HSV-2 or to these two viruses in serum of patients with cervical PVI were detected in 90.0 % of cases, in which the clinical symptoms of herpetic infection were no identificated. IgG antibodies to HSV-2 in serum of patients had only mean or low avidity. In patients with IgG antibodies to HSV-2 or simultaneously with HSV-2 and HSV-1, and, above all, with low- avidity IgG antibodies to HSV-2 the frequency of HPV-induced cervical intraepithelial neoplasia (CIN) of І-ІІ and ІІІ grade and cancer in situ was increased. The number of CD3⁺, CD4⁺, CD8⁺, CD4⁺45RA⁺ аnd CD16⁺/CD56⁺ cells in the patients’ peripheral blood, as well as the CD4⁺/CD8⁺ index were not altered compared to the indicators for clinically healthy subjects (control). But the tendency to increase the number of CD16⁺/CD56⁺/CD8⁺ cells, as well as a significant increase in the number of СD3⁺/HLA-DR⁺ cells were determined. The number of CD3⁺/CD16⁺/CD56⁺ cells was reduced in peripheral blood of patients with low-avidity of IgG antibodies to HSV-2, whereas in the remaining patients it was at the control level. meter FC-500 (Beckman Coulter, USA).

Results and discussion. In patients serum with CU HPVI without clinical signs of herpetic infection to HSV-1, HSV-2 IgG antibodies, both these two viruses simultaneously were diagnosed in 90.0 % of cases. Anti-HSV-2 IgG in patients’ serum had only medium or low avidity. The frequency of revealing HPV-induced cervical intraepithelial neoplasia (CIN) I-ІІ and ІІІ degree of activity, and cancer in situ increased in patients with anti-HSV-2 IgG or simultaneously to anti-HSV-1 and anti-HSV-2 but, first of all, in patients with low avidity anti-HSV-2 IgG. The number of CD3⁺, CD4⁺, CD8⁺, CD4⁺45RA⁺ and CD16⁺/CD56⁺ cells in the patients’ peripheral blood, as well as CD4⁺/CD8⁺ index did not change as compared with group of clinically healthy persons (control group). However, there was determined the tendency of CD16⁺/CD56⁺/CD8⁺ cells number increase and significant СD3⁺/HLA-DR⁺ cells number increase. CD3⁺/CD16⁺/CD56⁺ cells number in peripheral patients’ blood with low avidity to anti-HSV-2 IgG antibodies was reduced whereas it was equal to control level in the rest patients.

Conclusion. In patients with CU HPVI without clinical manifestation of herpetic infection anti-HSV-2 low avidity IgG were identified. The frequency of more severe CIN cause was increased. There were more significant displacement of cell immunity confirmed by CD3⁺/HLA-DR⁺ cells number increase and tendency of CD16⁺/56⁺CD8⁺ cells number increase, too, on the background of СD3⁺/CD16⁺/CD56⁺ cytotoxic T-lymphocytes decrease. Complex individual treatment of these patients with anti-herpetic drugs must probably be used.