PREVALENCE OF POLYMORPHISM OF THE TLR 9 TYPE GENE IN PATIENTS WITH CHRONIC FORMS OF INFECTIOUS MONONUCLEOSIS
DOI:
https://doi.org/10.11603/1681-2727.2018.1.8670Keywords:
chronic Epstein-Barr virus, Toll-like receptors, polymorphism, prevalence.Abstract
The aim of the study. To determine the TLR-914 T/C gene polymorphism in patients with CVEB caused by Epstein-Barr virus.
Materials and methods. Research for the determination of polymorphism -1486 T/C TLR-9 gene was performed in 44 patients with chronic forms of the VEB infection. A control group to study the prevalence of polymorphism -1486 T/C TLR-9 gene were 40 healthy donors. For detecting the DNA of the VEB by PCR with reverse transcription with hybridization and fluorescence detection of amplification products, the Amplisents reagent sets (Russia) were used. DNA isolation from the specimens was performed using a kit for DNA extraction by the Miniprep Company (Siles M, Russia), using the sorption assay technique of the sorbent. Polymorphic region -1486 T/C, rs187084 of the TLR9 gene was studied by real-time PCR amplification by determining the length of the restriction fragment-PCR using restriction enzymes Ncol and oligonucleotide primers.
Results. The analysis of the results of the -1486 T/C polymorphism of the TLR-9 gene revealed three main genotypes – TT, TC, CC. Investigation of the frequency of occurrence of individual genotypes revealed the dominance of the genotype TC, compared with the homozygous genotypes of TT and CC. The study of frequency distribution of the TLR -1486 T/C gene polymorphism for different genotypes demonstrated the specificity of changes in the TC genotype in patients with HEBV and the absence of such for the genotypes of TT and CC.
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