DETERMINATION QUANTITATIVE COMPOSITION OF THE MICROBIOTA IN PARIETAL INTESTINAL SURFACE IN RATS BY PCR REAL-TIME
DOI:
https://doi.org/10.11603/1681-2727.2016.3.6896Keywords:
parietal microflora, gut microbiota, vancomycin, PCR Real-time.Abstract
An important function of the intestinal microbiota is to protect the body against pathogens. Disruption of the balance leads to dysbiotic displays and increased susceptibility to bacterial infections. Of particular interest are the processes of interaction of vancomycin, Salmonella enteritidis and Salmonella typhimurium representatives on intestinal flora. The data obtained by PCR Real-time shows that when vancomycin is administered into the content of the intestine – decreased numbers of Bacteroides, Enterococci, Peptostreptococci were observed, along with increased numbers of Enterobacteria, Proteus, Klebsiella, Salmonella and Shigella. Administration of S.enteritidis and S.typhimurium reduced the number of E.coli and Bacteroides spp., while increasing the number of P.aeruginosa, E.faecalis, E.faecium, Enterobacter spp., Klebsiella spp. and Peptostreptococcus anaerobius. Therefore, there is still a need to further understand the processes of interaction between Salmonella enteritidis, Salmonella typhimurium and representatives of normal intestinal microflora under conditions of systemic antibiotic therapy. Additionally, it would be beneficial to investigate the formation of an immune response in the intestinal mucosa, in order to develop effective treatment regimens salmonellosis gastroenteritis etiology.References
Macpherson A.J. Interactions between commensal intestinal bacteria and the immune system / A.J. Macpherson, N.L. Harris // Nature Rev. Immunol. – 2004. – Vol. 4. – P. 478-485.
Deplancke B. Microbial modulation of innate defense: goblet cells and the intestinal mucus layer / B. Deplancke, H.R. Gaskins // Am. J. Clin. Nutrition. – 2001. – Vol. 73. – P. 1131-1141.
Human nutrition, the gut microbiome and the immune system / A.L. Kau, P.P. Ahern, N.W. Griffin [et al.] // Nature. – 2011. – Vol. 474. – P. 327-336.
Stecher B. The role of microbiota in infectious disease / B. Stecher, W.D. Hardt // Trends Microbiology. – 2008. – Vol. 16. – P. 107-114.
Vollaard E.J. Colonization resistance / E.J. Vollaard, H.A. Clasener // Antimicrobial Agents and Chemotherapy. – 1994. – Vol. 38. – P. 409-414.
Stecher B. Mechanisms controlling pathogen colonization of the gut / B. Stecher, W.D. Hardt // Curr. Opin. Microbiol. – 2011. – Vol. 14. – P. 82-91.
Salmonella typhimurium persists with in macrophages in the mesenteric lymph nodes of chronically infected Nramp1+/+ mice and can be reactivated by IFNgamma neutralization / D.M. Monack, D.M. Bouley, S.J. Falkow [et al.] // Experimental Medicine. – 2004. – Vol. 199. – P. 231-241.
Long-term impacts of antibiotic exposure on the human intestinal microbiota / C. Jernberg, S. Löfmark, C. Edlund [et al.] // Microbiology. – 2010. – Vol. 156. – P. 3216-3223.
Ubeda C. Antibiotics, microbiota and immune defense / C. Ubeda, E.G. Pamer // Trends Immunology. – 2012. – Vol. 33. – P. 459-466.
Differential effects of antibiotic therapy on the structure and function of human gut microbiota / A.E. Pérez-Cobas, A. Artacho, H. Knecht [et al.] // PLoS One. – 2013. – Vol. 8. – P. 201-208.
Antibiotics in early life alter the murine colonic microbiome and adiposity / I. Cho, S. Yamanishi, L. Cox [et al.] // Nature. – 2012. – Vol. 488. – P. 621-626.
Effect of various antibiotics on modulation of intestinal microbiota and bile acid profile in mice / Y. Zhang, P.B. Limaye, H.J. Renaud [et al.] // Toxicology and Applied Pharmacology. – 2014. – Vol. 277. – P. 138-145.
Role of the gut microbiota in defining human health / K.E. Fujimura, N.A. Slusher, M.D. Cabana [et al.] // Expert Rev. Anti-infective Therapy. – 2010. – Vol. 8. – P. 435-454.
Antibiotic treatment alters the colonic mucus layer and predisposes the host to exacerbated Citrobacter rodentium-induced colitis / M. Wlodarska, B. Willing, K.M. Keeney [et al.] // Infection and Immunity. – 2011. – Vol. 79. – P. 1536-1545.
Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability / P.D. Cani, S. Possemiers, T. Van de Wiele [et al.] // Gut. – 2009. – Vol. 58. – P. 1091-1103.
Effects on intestinal microflora during systemic antimicrobial prophylaxis in orthopaedic patients: Teicoplanin versus Cefazolin / E. Еrtazzoni Minelli, A. Benini, E. Barzoi [et al.] // Recent Advances in Chemotherapy. – 1992. – Vol. 1. – P. 1230-1231.
Life in the inflamed intestine, Salmonella style / R.L. Santos, M. Raffatellu, C.L. Bevins [et al.] // Trends Microbiol. – 2009. – Vol. 17. – P. 498-506.
Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome / I.M. Сarroll, Y.H. Chang, J. Park [et al.] // Gut Pathogenes. – 2010. – Vol. 2. – Р. 19.
Distinct microbial population exist in the mucosal-associated microbiota of subgroups of irritable bowel syndrome / G.C. Parkes, N.B. Rayment, B.N. Hudspith [et al.] // Neurogastroenterol. Motil. – 2012. – Vol. 24. – Р. 31-39.
Homeostatic regulation of Salmonella-induced mucosal inflammation and injury by IL-23 / M. Awoniyi, S.I. Miller, C.B. Wilson [et al.] // PloS One. – 2012. – Vol. 7. – Issue 5. – P. 1.
An obesity-associated gut microbiome with increased capacity for energy harvest / P.J. Turnbaugh, R.E. Ley, M.A. Mahowald [et al.] // PLoS One. – 2006. – Vol. 444. – P. 1027-1031.
Antibiotic-induced perturbations of the intestinal microbiota alter host susceptibility to enteric infection / I. Sekirov, N.M. Tam, M. Jogova [et al.] // PubMed. – 2008. – Vol. 76. – P. 4726-4736.
Lower Bifidobacteria counts in both – duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients / A.P. Kerckhoffs, M. Samson, M.E. van der Rest [et al.] // PloS One. – 2009. – Vol. 15. – Р. 2887-2892.
Short-term effect of antibiotics on human gut microbiota / S. Panda, I. Elkhader, F. Casellas [et al.] // PLoS One. – 2014. – Vol. 9. – P. 954-967.
Gut microbiota composition and development of atopic manifestations in infancy: the KOALA Birth Cohort Study / J. Penders, C. Thijs, P.A. Van den Brandt [et al.] // Gut. – 2007. – Vol. 56. – P. 661-667.
Downloads
Published
How to Cite
Issue
Section
License
Journal Infectious Disease (Infektsiini Khvoroby) allows the author(s) to hold the copyright without registration
Users can use, reuse and build upon the material published in the journal but only for non-commercial purposes
This journal is available through Creative Commons (CC) License BY-NC "Attribution-NonCommercial" 4.0
