ETIOLOGICAL STRUCTURE OF LYME BORRELIOSIS IN PATIENTS OF TERNOPIL REGION
DOI:
https://doi.org/10.11603/1681-2727.2023.1.13922Keywords:
Lyme borreliosis, diagnostics, immunoblotting, IgM, B. burgdorferi s. s., B. garinii, B. afzelii and B. spielmanii, clinicAbstract
SUMMARY. The aim – to find out the etiological structure of Lyme borreliosis in patients of Ternopil region by determining serum specific antibodies to B. burgdorferi s. s., B. garinii, B. afzelii and B. spielmanii.
Material and Methods. The study was conducted in 2019-2022 at the Ternopil Regional Clinical Hospital and the Ternopil Regional Clinical Dermatology and Venereology Dispensary. Depending on the involvement of certain organs and systems, they were divided into two groups: group 1 – 20 people with cardiovascular disease and group 2 – 19 patients with damage to other organs and systems. A total of 39 patients with LB aged 18 to 71 years were 56.4 % male (22) and 43.6 % female (17). They were treated on an outpatient and inpatient basis. The LB was diagnosed by ICD-10, according to which the disease belongs to the subsection «Other infections caused by spirochetes» and is coded as A69.2. LB was evaluated by two-tier testing (ELISA and immunobloting). The specific antibodies to the B complex. Burgdorferi s.l. complex in the patients’ blood sera was detected: class M antibodies – by the Anti-Borrelia burgdorferi ELISA (IgM) test system; class G – by Anti-Borrelia plus VIsE ELISA (IgG), test systems from EUROIMMUN AG (Germany). For the detection of only IgM against OspC antigens of four species of Borrelia simultaneously (B. burgdorferi s.s., B. garinii, B. afzelii and B. spielmanii) a line blot assay was applied using the EUROLINE Borrelia RN-AT adv.
Results. In patients with Lyme borreliosis it was possible to detect IgM to the OspC antigen of four Borrelia genera: B. spielmanii, B. burgdorferi s.s., B. garinii and B. afzelii – using the EUROLINE Borrelia RN-AT adv. line blot. The results of this assay allowed to expand the pathogenic range of LB in the Ternopil region. Specific IgM against the OspC antigen of Borrelia borrelii of four genera (B. burgdorferi s. s., B. garinii B. afzelii and B. spielmanii) were detected simultaneously only in the sera of 21.1% of patients with Lyme borreliosis with cardiovascular damages.
Conclusions. It has been established that separately or in combination Borrelia of four genotypes such as: B. burgdorferi s. s., B. garinii B. afzelii and B. spielmanii – are involved in the occurrence of Lyme borreliosis in patients with damage of the cardiovascular system and other organs and systems.
References
Bron, G. M., Fenelon, H., & Paskewitz, S. M. (2021). Assessing Recognition of the Vector of Lyme Disease Using Resin-Embedded Specimens in a Lyme Endemic Area. Journal of medical entomology, 58(2), 866–872. https://doi.org/10.1093/jme/tjaa234 DOI: https://doi.org/10.1093/jme/tjaa234
Radolf, J. D., Strle, K., Lemieux, J. E., & Strle, F. (2021). Lyme Disease in Humans. Current issues in molecular biology, 42, 333–384. https://doi.org/10.21775/cimb.042.333 DOI: https://doi.org/10.21775/cimb.042.333
Karvonen, K., Nykky, J., Marjomäki, V., & Gilbert, L. (2021). Distinctive Evasion Mechanisms to Allow Persistence of Borrelia burgdorferi in Different Human Cell Lines. Frontiers in microbiology, 12, 711291. https://doi.org/10.3389/fmicb.2021.711291 DOI: https://doi.org/10.3389/fmicb.2021.711291
Bobe, J. R., Jutras, B. L., Horn, E. J., Embers, M. E., Bailey, A., Moritz, R. L., Zhang, Y., Soloski, M. J., Ostfeld, R. S., Marconi, R. T., Aucott, J., Ma’ayan, A., Keesing, F., Lewis, K., Ben Mamoun, C., Rebman, A. W., McClune, M. E., Breitschwerdt, E. B., Reddy, P. J., Maggi, R., … Fallon, B. A. (2021). Recent Progress in Lyme Disease and Remaining Challenges. Frontiers in medicine, 8, 666554. https://doi.org/10.3389/fmed.2021.666554 DOI: https://doi.org/10.3389/fmed.2021.666554
Sykes, R. A., & Makiello, P. (2017). An estimate of Lyme borreliosis incidence in Western Europe†. Journal of public health (Oxford, England), 39(1), 74–81. https://doi.org/10.1093/pubmed/fdw017 DOI: https://doi.org/10.1093/pubmed/fdw017
Andreichyn M. A., Kopcha V. S. & Shkilna M. I. (2019). Lyme borreliosis. Diagnostic criteria, treatment and prevention: Method. Recommendations. Ternopil, TDMU, 52 [in Ukrainian].
Chemych, M., & Lutai, I. (2020). Lyme disease. Modern issue condition. Eastern Ukrainian Medical Journal, 8(2), 230-241 [in Ukrainian]. DOI: https://doi.org/10.21272/eumj.2020;8(2):230-241
Vasylieva, N. A., Ivakhiv, O. L. & Kachor, V. O. (2011). Lyme disease in Ternopil region. Infektsiini khvoroby – Infectious diseases, 2, 50–53 [in Ukrainian].
Mancilla-Agrono, L. Y., Banguero-Micolta, L. F., Ossa-López, P. A., Ramírez-Chaves, H. E., Castaño-Villa, G. J., & Rivera-Páez, F. A. (2022). Is Borrelia burgdorferi Sensu Stricto in South America? First Molecular Evidence of Its Presence in Colombia. Tropical medicine and infectious disease, 7(12), 428. https://doi.org/10.3390/tropicalmed7120428 DOI: https://doi.org/10.3390/tropicalmed7120428
Trevisan, G., Cinco, M., Trevisini, S., di Meo, N., Chersi, K., Ruscio, M., Forgione, P., & Bonin, S. (2021). Borreliae Part 1: Borrelia Lyme Group and Echidna-Reptile Group. Biology, 10(10), 1036. https://doi.org/10.3390/biology10101036 DOI: https://doi.org/10.3390/biology10101036
Nebohatkin, I. V. & Shulhan A. M. (2020). Epidemiological and epizootic features of Lyme disease in 2019 in Ukraine. Aktualna infektolohiia – Actual infectology, 8 (5/6), 57–61 [in Ukrainian]. DOI: https://doi.org/10.22141/2312-413x.8.5-6.2020.217959
Turianytsia, S. M., Andrashko, Yu. V., Petrov, V. O. & Sakal, M. M. (2012). Dynamics of the Lyme disease situation in Transcarpathia]. Klinichna imunolohiia. Alerholohiia. Infektolohiia – Clinical immunology. Allergology. Infectology, 1–2 (50–51), 61–64 [in Ukrainian].
Coipan, E. C., Jahfari, S., Fonville, M., Oei, G. A., Spanjaard, L., Takumi, K., Hovius, J. W., & Sprong, H. (2016). Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 42, 66–76. https://doi.org/10.1016/j.meegid.2016.04.019 DOI: https://doi.org/10.1016/j.meegid.2016.04.019
Sprong, H., Azagi, T., Hoornstra, D., Nijhof, A. M., Knorr, S., Baarsma, M. E., & Hovius, J. W. (2018). Control of Lyme borreliosis and other Ixodes ricinus-borne diseases. Parasites & vectors, 11(1), 145. https://doi.org/10.1186/s13071-018-2744-5 DOI: https://doi.org/10.1186/s13071-018-2744-5
Rudenko, N., Golovchenko, M., Grubhoffer, L., & Oliver, J. H., Jr (2011). Updates on Borrelia burgdorferi sensu lato complex with respect to public health. Ticks and tick-borne diseases, 2(3), 123–128. https://doi.org/10.1016/j.ttbdis.2011.04.002 DOI: https://doi.org/10.1016/j.ttbdis.2011.04.002
Shkilna, M. I. (2021). Clinical and epidemiological and immunological aspects of Lyme borreliosis, improvement of diagnosis and therapy: PhD dissertation: 14.01.13. Ternopil, 436 [in Ukrainian].
Zadorozhna, V. I., Rudenko, A. O., Klius, V. Yu. (2017). Lyme borreliosis is a particularly dangerous infection. Threats and risks. Veterynarna medytsyna – Veterinary medicine, 103, 30–32 [in Ukrainian].
Kozlovska, A. (2020). Lyme-borreliosis: modern principles of treatment from A to Z. Klinichna imunolohiia. Alerholohiia. Infektolohiia – Clinical immunology. Allergology. Infectology, 5 (126), 34–39 [in Ukrainian].
Baker P. J. (2019). Is It Possible to Make a Correct Diagnosis of Lyme Disease on Symptoms Alone? Review of Key Issues and Public Health Implications. The American journal of medicine, 132(10), 1148–1152. https://doi.org/10.1016/j.amjmed.2019.04.001 DOI: https://doi.org/10.1016/j.amjmed.2019.04.001
Chou, E., Minor, A., & Cady, N. C. (2021). Quantitative multiplexed strategies for human Lyme disease serological testing. Experimental biology and medicine (Maywood, N.J.), 246(12), 1388–1399. https://doi.org/10.1177/15353702211003496 DOI: https://doi.org/10.1177/15353702211003496
Branda, J. A., Strle, F., Strle, K., Sikand, N., Ferraro, M. J., & Steere, A. C. (2013). Performance of United States serologic assays in the diagnosis of Lyme borreliosis acquired in Europe. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 57(3), 333–340. https://doi.org/10.1093/cid/cit235 DOI: https://doi.org/10.1093/cid/cit235
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 I. S. Kovbasyuk
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Journal Infectious Disease (Infektsiini Khvoroby) allows the author(s) to hold the copyright without registration
Users can use, reuse and build upon the material published in the journal but only for non-commercial purposes
This journal is available through Creative Commons (CC) License BY-NC "Attribution-NonCommercial" 4.0
