SEROLOGICAL DIAGNOSTIC OF TICK-BORNE INFECTIONS IN PATIENTS WITH LOCALIZED SCLERODERMA

Authors

DOI:

https://doi.org/10.11603/1681-2727.2021.3.12490

Keywords:

localized scleroderma, Lyme borreliosis, tick-borne relapsing fever, bartonellosis

Abstract

The aim of the study was to determine the frequency of detection of specific IgM and/or IgG antibodies to Borrelia burgdorferi s. l., B. miyamotoi, Bartonella henselae and Bartonella quintana in the patient’s serum with localized scleroderma.

Material and methods. There were 78 patients with localized scleroderma aged 18–74 under supervision; in 2015–2021 they were on outpatient and inpatient treatment at Ternopil Regional Clinical Dermatological and Venereological Dispensary. There were 17 men (21.8 %) and 61 women (78.2 %). To detect specific anti-IgM and/or anti-IgG to B. burgdorferi s. l. (causative agent of Lyme- borreliosis) in the blood serum of patients a two-step algorithm of serological diagnosis of LB (ELISA and immunoblot) by the Euroimmun AG (Germany) test systems was used. The obtained results were analysed according to the manufacturer’s recommendations. IgM and IgG antibodies to B. miyamotoi (one of the causative agents of tick-borne relapsing fever (TBRF) were determined in the serum by immunoblotting in the IGeneX Inc. laboratory (Milpitas, California, USA). Specific IgG antibodies to Bartonella henselae and Bartonella quintana (causative agent of bartonellosis) were determined in the serum of patients by the multiplex indirect immunofluorescence method, Mosaic for Bartonella henselae/Bartonella quintana IgG test system, Euroimmun AG (Germany), using BIOCHIP technology, which contained fluorescein-labelled antigens of these species of bartonella.

Results. Positive or intermediate antibodies of at least one IgM and/or IgG class to the complex of B. burgdorferi s. l. were detected in 29 (37.2 %) of 78 patients with localized scleroderma. The data obtained by immunoblotting were confirmed in 25 (86.2 %) patients. Antibodies of the IgM class simultaneously to B. burgdorferi s.l. and B. miyamotoi were diagnosed by immunoblotting in 4 (11.1 %), anti-IgG y – in 5 (13.9 %) of 36 patients with localized scleroderma. Multiplex indirect immunofluorescence method allows diagnosis IgG antibodies only to Bartonella henselae in the serum of 4 (15.3 %) of 26 patients with localized scleroderma. Serological diagnosis of tick-borne relapsing fever (TBRF) and bartonellosis (present or in the past) in the patients with localized scleroderma, residents of Ternopil region, was conducted for the first time.

Conclusions. The use of a two-step algorithm of serological diagnosis of LB (ELISA and immunoblot) revealed IgM and/or IgG antibodies to B. burgdorferi s. l. in 32.1 % of patients with localized scleroderma. The involvement of B. miyamotoi into clinical manifestations of localized scleroderma in 13.9 % of patients by detecting IgG antibodies to both B. miyamotoi and B. burgdorferi s. l. by immunoblotting was established. Specific IgG antibodies to Bartonella henselae alone were diagnosed in the serum of 15.3 % of patients with localized scleroderma.

Author Biographies

K. B. Shtokailo, I. Horbachevsky Ternopil National Medical University

PhD student of the Department of Infectious Diseases and Epidemiology, Dermatology and Venerelogy, I. Horbachevsky Ternopil National Medical University

J. C. Cruz, Laboratory “IGeneX Inc.” (Milpitas, CA, USA)

Clinical Laboratory Director, «IGeneX Inc.» (Milpitas, California, USA)

I. D. Cruz, Laboratory “IGeneX Inc.” (Milpitas, CA, USA)

Clinical Laboratory Manager, «IGeneX Inc.» (Milpitas, California, USA)

M. A. Andreychyn, I. Horbachevsky Ternopil National Medical University

academician of the NAMS of Ukraine, Professor, MD, Head of the Department of Infectious Diseases and Epidemiology, Skin and Venereal Diseases, I. Horbachevsky Ternopil Medical University

M. I. Shkilna, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor of the Department of Infectious Diseases and Epidemiology, Dermatology and Venerelogy, I. Horbachevsky Ternopil National Medical University

O. L. Ivahiv, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor of Department of Infectious Diseases with Epidemiology, Dermatology and Venerelogy, I. Horbachevsky Ternopil National Medical University

M. M. Korda, I. Horbachevsky Ternopil National Medical University

MD, Professor, Rector, I. Horbachevsky Ternopil National Medical University

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Published

2021-11-29

How to Cite

Shtokailo, K. B., Cruz, J. C., Cruz, I. D., Andreychyn, M. A., Shkilna, M. I., Ivahiv, O. L., & Korda, M. M. (2021). SEROLOGICAL DIAGNOSTIC OF TICK-BORNE INFECTIONS IN PATIENTS WITH LOCALIZED SCLERODERMA. Infectious Diseases – Infektsiyni Khvoroby, (3), 33–42. https://doi.org/10.11603/1681-2727.2021.3.12490

Issue

No. 3 (2021)

Section

Original investigations

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