LYME BORRELIOSIS AND TICK-BORNE RELAPSING FEVER IN FORESTERS OF TERNOPIL REGION
DOI:
https://doi.org/10.11603/1681-2727.2020.2.11283Keywords:
Lyme borreliosis, tick-borne relapsing fever, diagnostics, ELISA, immunoblot, questionnaire, forestry workersAbstract
The aim of the study – to determine the case incidence of B. burgdorferi s. l. (the agent of Lyme Borreliosis) and B. hermsii та B. miyamotoi (the agents of tick-borne relapsing fever) infection among foresters of the Ternopil region and study the clinical manifestation of combined dual infection using of two-step diagnostic scheme (ELISA and immunoblotting).
Patients and Methods. 339 foresters were involved in the study in the Ternopil region: 4 forestries (Buchah, Krementz, Ternopil, and Chortkiv districts) and Berezhany’s forestry and hunting grounds. The age of patients was from 20 to 65 years old. There were 330 males and 9 females. Antibodies to B. burgdorferi s. l. antigens were detected by Euroimmun AG assay (Germany). Serum samples with positive and borderline results were confirmed by immunoblot (EUROLINE Borrelia RNAT). All tests were done in the Center of Study of Lyme borreliosis and other infectious diseases transmitted by ticks at I. Horbachevsky Ternopil National Medical University.
For detection of antibodies to B. hermsii та B. miyamotoi – the cause of tick-borne relapsing fever, we use 114 human blood samples of forestry workers, which were positive or borderline to B. burgdorferi s. L IgM and IgG antibodies in the ELISA test. The average age of involved persons was from 20 to 65 years old, among them 113 males and 1 female. Samples were examined in the laboratory IGeneX Inc. (Milpitas, CA, USA) by immune blotting.
Results. Immunoblot examination confirmed the presence of B. burgdorferi s. l (B. burgdorferi s. s., B. afzeliі and B. garinii) IgM and/or IgG antibodies (positive or borderline results) in 85.1 % of 114 forestry workers of Ternopil region. The first time, IgM and/or IgG antibodies to agents of tick-borne relapsing fever (B. hermsii та B. miyamotoi) were detected by immunoblot in 34.2 % of 114 forestry workers of Ternopil regions, among those samples which were founded previously positive B. burgdorferi s. l IgM and/or IgG antibodies by ELISA enzyme immunoassays.
The IgG antibodies to Lyme Borreliosis pathogens were detected more frequently – 78.1 versus 21.9 %, p<0.05, while to tick-borne relapsing fever pathogens only in 12.3 versus 85.9 % (p<0.05) of cases. B. burgdorferi s. l. IgG-antibodies were detected more frequently compared to B. hermsii and B. miyamotoi IgG-antibodies (78.1 versus 12.3 %, p<0.05). Forestry workers which were positive to antibodies to both Lyme borreliosis and tick-borne relapsing fever, noticed earlier beginning of tick-bites’ season (March-October). They manifested intoxication syndrome and musculoskeletal damage more frequently compared to persons which were positive only to B. burgdorferi s. l. antibodies.
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