Influence of intra-abdominal pressure and ischemically-reperfusive losses on the manifestation of cytolytic syndrome and its correction by thiocetam in the experiment
DOI:
https://doi.org/10.11603/2414-4533.2018.2.9216Keywords:
intra-abdominal pressure, ischemic-reperfusion syndrome, cytolysis, thiocetam.Abstract
The aim of the work: to determine the effect of ischemia-reperfusion disorders on the manifestations of cytolysis syndrome depending on the magnitude of intra-abdominal pressure and the effectiveness of correcting the revealed disorders with thiocetam.
Materials and Methods. In the experiment, 90 mature white Wistar male rats weighing 200–250 g were used, which were on a standard ration of the vivarium. All experimental animals were anesthetized (thiopental sodium 40 mg • kg-¹ intraperitoneally) and modeled an increase in intra-abdominal pressure by 10, 20 and 30 mm Hg by injecting into the abdominal cavity of atmospheric air. The exposure time was 90 minutes, after which the control animals and animals of each study group were immediately withdrawn from the experiment by total bloodletting from the heart. The other animals of each study group (6 specimens each) under anesthesia (thiopental sodium 40 mg-kg-¹ intraperitoneally) were withdrawn from the experiment 1, 3 and 7 days after reperfusion. In individual groups, the animals were injected intraperitoneally with Thioacetam (Galichpharm, Ukraine) daily at a dose of 250 mg kg-1 intraperitoneally after 6 days of reperfusion. From the expert they were taken out after 7 days. The animals were collected blood, in the serum of which the activity of indicator cytolysis enzymes alanine and aspartate aminotransferase (ALT, AST) was determined by a unified method for the biochemical analyzer Humalyzer 2000.
Results and Discussion. Simulation of increased intra-abdominal pressure for 90 min is accompanied by a marked cytolytic syndrome, manifested by a significant increase in serum ALT activity. The greatest disturbances occur after reperfusion with a value of increased intra-abdominal pressure of 20 and 30 mm Hg. In these conditions, the activity of ALT in the blood serum increases even more, there is an increase in ASAT activity with a maximum in 3–7 days. Use with the corrective purpose of thiocetam at a dose of 250 mg • kg-¹ intraperitoneally for 6 days is accompanied by a pronounced positive effect, which is manifested by a significant decrease in the activity of ALAT and ASAT in the blood serum with intra-abdominal pressure of 20 and 30 mm Hg.
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