Features of activation of lipid peroxidation processes in conditions of cranioskeletal injury in rats of different age
DOI:
https://doi.org/10.11603/2414-4533.2018.2.9213Keywords:
cranioskeletal trauma, experiment, age, lipid peroxidation.Abstract
The aim of the work: to find out the content of TBK-active liver oil products in the dynamics of cranioskeletal trauma in rats of different age groups.
Materials and Methods. In experiments on nonlinear white rats males of three age groups (100 to 120 days old, sexually mature animals aged 6–8 months, and old animals aged 19–23 months) under the conditions of thiopetalonatrioneous anesthesia modeled a cranioskeletal trauma by measuring a skeletal equivalent to animals different age groups according to the severity of the defeat, which caused a closed craniocerebral trauma of moderate severity. In addition, a closed fracture of the left femur was modeled by a metered blow. Animals that survived in the conditions of thiopental-sodium excitement (80 mg • kg-1 body weight intraperitoneally) after 1, 3, 7, 14, 21 and 28 days after trauma were withdrawn from the experiment by the method of total blood-flow from the heart. The liver tissue evaluated the activity of lipid peroxidation processes on the content of TBK-active products of LPP.
Results and Discussion. Modeling of cranioskeletal trauma is accompanied by activation of lipid peroxidation processes in the liver in individuals of different ages, which lasted in time and did not reach control level for 28 days. In the immature individuals, as a result of cranioskeletal trauma, up to 3 days, the content of TBK-active products of LPO in the liver increases more slowly than in individuals of other age groups, however, after 7 days, the antioxidant defense mechanisms become depleted and the rate of growth of the indicator becomes the largest. In the future, due to the integration of long-term adaptation mechanisms, the indicator decreases more intensively and becomes less likely than in other age groups. The dynamics of the content of TBC-active liver LPO in the liver in the mature and elderly rats during the first 14 days of the post-traumatic period is similar to a rapid increase in the index within 1 day of the experiment and reaches a maximum of 7 days, which practically did not change to 14 days. In the future, the indicator is declining, but in mature animals more intensive than in the old. The results obtained should be taken into account when developing experimental methods for correction of traumatic illness with regard to age.
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