Peculiarities of local immune reactions in jejunum at postresection portal hypertension
DOI:
https://doi.org/10.11603/2414-4533.2018.2.9201Keywords:
postresection portal hypertension, jejunum, local immunity.Abstract
The aim of the work: to investigate the features of local immune reactions in the je junum at postresection portal hypertension.
Materials and Methods. The research was conducted on 45 male rats, which were divided into 3 groups. The group 1 consisted of 15 intact animals, 2–15 rats after resection of the left lateral part – 31.5 % of liver parenchyma, 3–15 animals after resection of the right and left side parts of the liver (58.1 %). Euthanasia of animals was carried out by bloodletting in conditions of thiopental anesthesia 1 month after the beginning of the experiment. The cut pieces of the jejunum were fixed in a 10 % neutral formalin solution, and after appropriate ethyl alcohol of increasing concentration was poured in paraffin blocks. Histologic sections 5–7 mm thick after deparaffinization were stained with hematoxylin-eosin, for van Gizon, Mallory, Weigert, and toluidine blue. In the detection of plasmocytes with IgA, IgM, IgG, IgE histologic sections of the jejunum was treated with monospecific antiserum against these classes of immunoglobulins conjugated with fluorescein isothiocyanate using the direct Kuns method with appropriate controls that were studied using a luminescent microscope “Lumam P-8”. In a luminescent light, plasmatic cells that gave a specific luminescence were read on 1 mmІ of the mucosa of the investigated organ. The content of secretory Ig A (SIgA) was determined by the method of separate immunodiffusion in agar with a specific serum against SIgA. Quantitative indicators were processed statistically.
Results and Discussion. One month after resection of 31.5 % of the liver, local immune reactions in the mucosa of the jejunum changed slightly. Violation of local immune homeostasis was more pronounced in resection of 58.1 % of the liver parenchima. Thus, the number of plasmatic cells with IgA in the mucosa of the subject decreased by 26.6 %, the level of secretory immunoglobulin A decreased by 27.9 %, while the plasmocytes producers IgM, IgG, IgE increased accordingly by 70.1 %, 3.8 and 4.6 times. The degree of changes in local homeostasis correlated with the depth and prevalence of hemodynamic disorders, dystrophic, fibrotic, infiltrative and sclerotic processes in the investigated organ, that is, it plays an important role in the morphogenesis of the jejunum at postresection portal hypertension. Accordingly, resection of 58.1 % of liver parenchyma leads to postresection portal hypertension and pronounced changes in local immune homeostasis in the jejunum mucosa, characterized by a decrease in the levels of SIgA and the number of IgA-producing plasmocytes, significant violations of the relation between the immunocytes that synthesize IgA, IgM, IgG, IgE, the emergence of immune complexes in the stroma and wall of vessels. The degree of changes in local immune homeostasis correlates with the depth and prevalence of hemodynamic disorders, dystrophic, fibrotic, infiltrative and sclerotic processes in the investigated organ.
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