Influence of modified gastric plication and gastric sleeve resection in rats with glutamate-induced obesity and streptozotocin-induced diabetes on the dynamics of body weight loss and hydrocarbon metabolism
DOI:
https://doi.org/10.11603/2414-4533.2018.1.8863Keywords:
gastric plication (GP), sleeve gastric resection (SGR), morbid obesity, diabetes mellitus.Abstract
The aim of the work: to compare the influence of modified gastric plication and gastric resection in rats with glutamate-induced obesity and streptozotocin-induced diabetes on the dynamics of body weight and hydrocarbon metabolism.
Materials and Methods. The study was probed on 45 white, non-breeding female rats with an initial body weight of 150–180 grams. Glutamate-induced obesity and streptotrozine-induced diabetes were modeled on animals. Animals were divided into 3 groups with 15 animals in each control group, and two experimental groups. In the first experimental group we performed sleeve resection of the stomach; in the second one plication of the stomach on a large curvature with the formation of 3 folds in the first row of sutures and the second series of continuous suture. After that, a mass of animals and glucose were determined on the 14th, 28th, 42nd and 60th day.
Results and Discussion. The level of glucose in the control group on the 60th postoperative day was (18.405 ±1.2) mmol/l, in the first experimental group, where the sleeve resection of the stomach was made, it was (9.539±1.14) mmol/l, in the second group, where the modified gastric plication was made, was (10.39 ±1.49) mmol / l. The determination of glucose in serum on the 60th day after the operation established high statistical significance in decrease of serum glucose between the control and the first experimental group where a sleeve resection was performed (p <0.01) and a high statistical significance in level of serum glucose reduction was observed between the control group and the second experimental group, where a modified stomach plication technique was performed (p <0.01). However, no statistically significant difference between the first and second experimental groups was found (p>0.05).
The weight of the body in the control group on the 60th day was (335±17.32) g, in the first experimental group it was on the 60th day (260±12.96) g and in the second experimental group (258.33±11.44) g. The determination of the body weight of rats found a statistically significant decrease in body weight on the 60th postoperative day in rats, which was performed on the sleeve resection of the stomach in comparison with the control group (p <0.01) and a statistically significant reduction in body weight in rats on the 60th post operative day after modificated gastric plication in comparison with the control group (p <0.01). However, the statistically significant difference between the weight of the body in rats of the first and second experimental groups on the 60th postoperative day was not revealed (p>0.05).
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