Quantitative morphological analysis of features of arteries remodeling of duodenum at different volume resections of liver parenchyma
DOI:
https://doi.org/10.11603/2414-4533.2017.4.8332Keywords:
resection of the liver, duodenum, arteries, remodeling.Abstract
The aim of the work: quantitative morphological methods to study the features of remodeling of the arteries of the duodenum at resection of different volumes of liver parenchyma.
Materials and Methods. The complex of morphological methods examined the duodenum of 43 sexually mature white male rats, which were divided into 4 groups. The first group consisted of 12 intact animals, second – 11 rats, in which 31.5 % of liver parenchyma was removed, third – 12 animals, which removed 42 % of liver parenchyma, 4–8 rats after resection 58, 1 % liver parenchyma. Euthanasia of experimental animals was carried out by bloodletting in conditions of thiopental anesthesia 1 month after the beginning of the experiment. From the duodenum histological preparations were made. The morphometry of arteries of small caliber (26–50) microns of duodenum was measured, in which their external and internal diameters, the thickness of the media and adventitia, the height of the endothelial cells, the diameter of their nuclei were determined, the Wogenworth index, the nuclear-cytoplasmic ratios in the endothelial cells and the relative volume damaged endothelial cells. Quantitative indicators were processed statistically.
Results and Discussion. It was established that during resection of 31.5 % of liver parenchyma morphometric indices studied were insignificantly changed. When removing 42 % of liver parenchyma, the outer diameter of these vessels increased statistically significantly (p <0.001) by 8.1 %, while the thickness of the media and adventia was 21.3 % and 8.6 %, respectively, and the index Wogenworth increased 1.76 times, and the internal diameter decreased by 18.5 %. The morphometric parameters of endothelial cells and their nuclei, which resulted in marked changes in their correlation between the nucleus and the cytoplasm, evidenced the disruption of cellular structural homeostasis, were unevenly and disproportionally altered. The most pronounced changes in the examined vessels were detected one month after the removal of 58.1 % of liver parenchyma. At the same time, the outer diameter of small-caliber arteries has increased statistically significantly (p <0.001) by 9.5 %, the thickness of the media is by 36.9 %, the thickness of the adventitia is by 44.8 %, the Wogenworth index is 2.66 times, indicating significant violation of the capacity of the examined vessels. The internal diameter of small-caliber arteries with a significant statistical significance (p <0.001) decreased by 31.4 %. The height of the endothelial cells of the vessels was changed by 13.0 %, and their nuclei – by 8.4 % (p <0.001). The nuclear-cytoplasmic relationships in the endothelial cells of the studied arteries were significantly disturbed, and the relative volume of damaged endothelial cells increased by 15.9 times. The presented evidence shows a pronounced remodeling of arteries of the duodenum in the removal of 42 and 58.1 % of liver parenchyma, which leads to deterioration of blood supply to the organ, hypoxia, dystrophy, necrobiosis of cells and tissues, and later infiltrative and sclerotic processes.
References
Avtadnilov, G.G. (2002). Osnovy kolichestvennoy patologicheskoy anatomii [Basis of quantitative pathological anatomy]. Moscow: Meditsina [in Russian].
Vishnevskiy, V.A., Yefanov, M.G., & Kazakov, I.V. (2012). Segmentarnyye rezektsyi, otdaliennyye rezultaty pri zlokachestvennykh opukholyakh pyechieni [Segmentar resections, long-term results in malignant liver tumors]. Ukr. Zhurnal Khirurgii – Ukrainian Journal of Surgery, 1 (16), 5-15 [in Ukrainian].
Hnatiuk, M.S., Tatarchuk, L.V., & Daniv, M.V. (2009). Morfometrychna otsinka vikovykh osoblyvostei remodeliuvannia arterii dvanadtsiatypaloi kyshky [Morphometric evaluation of the features of remodeling arteries of duodenum]. Klinichna anatomiia ta operatyvna khirurhiia – Clinical Anatomy and Operative Surgery, 8, (4) 54-57 [in Ukrainian].
Hnatiuk, M.S., Tatarchuk, L.V., & Yasinovskyi, O.B. (2016). Morfometrychna otsinka osoblyvostei remodeliuvania struktur dvanadtsiatypaloi kyshky pry resektsii riznykh obiemiv pechinky [Morphometric evaluation of the features of remodeling of duodenal structures during resection of different volumes of the liver]. Naukovyi visnyk Uzhhorodskoho universytetu. Seriia “Medytsyna” – Scientific Journal of Uzhhorod University. Series "Medicine", 1 (49), 3-5 [in Ukrainian].
(2003). Zahalni etychni pryntsypy eksperymentiv na tvarynakh [General ethical principles of experiments on animals]. Endokrynolohiia – Endocrinology, 8, 1, 142-145 [in Ukrainian].
Lapach, S.N., Gubenko, A.V., & Babich, P.N. (2001). Statistichieskiye metody v mediko-biologicheskikh issledovaniyakh Excell [Statistical methods in medicobiological investigations Excell]. Kyiv: Morion [in Russian].
Makarov, M.A., Avdyeyev, S.G., Chuchalin, A.G. (2012). Rol disfunktsyi endoteliya i regidnosti arteriy v patogeneze khronicheskoy obstruktivnoy boliezni liegkikh [Role of disfunction of endothelial cell in medical and biological investigation]. Terapevtichiskiy arkhiv – Theraputical Archive, 3, 74-80 [in Russian].
Sarkisov, D.S. (1998). Strukturnyye osnovy adaptatsyi i kompensatsyi narushennykh funktsyy [Structural basis adaptation and compensation damage function]. Moscow: Meditsina [in Russian].
Sorochinnikov, A.G., & Dorosyevich, A.Ye. (2007). Gistologichieskaya i mikroskopicheskaya tekhnika [Histological and microscopic equipments]. Moscow: Meditsina [in Russian].
Fodorov, V.D., Vishnyevskiy, V.A. & Nazarenko, N.A. (2007). Osnovnye oslozhnieniya obshyrnykh rezektsyy pecheni i puti ikh preduprezhdeniya [The main complications of extensive liver resections and ways to prevent them]. Byull. Sibirskoy mieditsyny – Journal of Siberian Medicine, 4, 16-24 [in Russian].
Shormanov, S.V., & Kulikov, S.V. (2010). Gistologichieskiye i ultrastrukturnye izmineniya pecheni pri ekspierimentalnom stenoze legochnogo stvola na stadii dekompensatsyi [Histological and ultrastructural changers at experimental stenosis of pulmonary trunk at insufficiency]. Mofrologiya – Morphology, 3, 46-50 [in Russian].
Nanashima, A., Sumida, Y., & Abo, T.(2008). A modified grading system for post-hepatectomy metastatic liver сancer originating form colorectal carcinoma. J. Surg. Oncol., 98, 363-370.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish their work in Hospital Surgery. Journal by L. Ya. Kovalchuk agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
