Apo-1/fas, ctla-4 and bcl-2 genes polymorphism in patients, operated on nodular thyroid pathology

Authors

  • M. I. Sheremet Bukovynian State Medical University, Chernivtsi
  • L. P. Sydorchuk Bukovynian State Medical University, Chernivtsi
  • V. O. Shidlovskyi I. Horbachevsky Ternopil State Medical University
  • A. D. Bedeniuk I. Horbachevsky Ternopil State Medical University
  • G. S. Kurochkin State University of Medicine and Pharmacy “Nicolae Testemitanu” Chisinau, Moldova
  • A. V. Levitsky State University of Medicine and Pharmacy “Nicolae Testemitanu” Chisinau, Moldova

DOI:

https://doi.org/10.11603/2414-4533.2017.2.7949

Keywords:

nodular goiter on the background of autoimmune thyroiditis, thyroid adenoma, polymorphisms APO-1/Fas, CTLA-4 and BCL-2 genes.

Abstract

The aim of the work: to analyze the frequency of polymorphic variants of genes BCL-2 (rs17759659), CTLA-4 (rs231775), APO-1/ Fas (rs2234767) in patients, operated on nodular thyroid pathology with regard to its form (NGAIT, TA- thyroid adenoma).

Materials and Methods. An analysis of the frequency of polymorphic variants of genes BCL-2 (rs17759659), CTLA-4 (rs231775), APO-1/Fas (rs2234767) in 125 patients, operated on nodular thyroid pathology with regard to its form (NGAIT, TA) was conducted. Also, we examined 25 healthy donors. The study of polymorphism of genes was carried out by the method of polymerase chain reac­tion in real time.

Results and Discussion. Mutation of BCL-2 gene (rs17759659) and CTLA-4 (rs231775) in the homozygous state among patients, operated on nodular thyroid pathology occurs with a frequency 3.2–4.0 % no reliable difference between patients with thyroid pathology and healthy. Mutation of the gene APO-1/Fas (rs2234767) in the homozygous state among surveyed patients was not met at all.

Author Biographies

M. I. Sheremet, Bukovynian State Medical University, Chernivtsi

1.  к. мед. н., доцент кафедри хірургії №1 БДМУ;

Адреса - м. Чернівці, вул. Головна, 137

L. P. Sydorchuk, Bukovynian State Medical University, Chernivtsi

1.  проф., д. мед. н., професор, зав. кафедри сімейної медицини БДМУ

V. O. Shidlovskyi, I. Horbachevsky Ternopil State Medical University

1.  д. мед. н., професор кафедра хірургії №1 з урологією та малоінвазивною хірургією імені професора Л.Я. Ковальчука ВНЗ «Тернопільський державний медичний університет ім. І.Я.Горбачевського»

Адреса – м. Тернопіль, Майдан Волі, 1

A. D. Bedeniuk, I. Horbachevsky Ternopil State Medical University

1.  д. мед. н., професор, зав. кафедра хірургії №1 з урологією та малоінвазивною хірургією імені професора Л.Я. Ковальчука ВНЗ «Тернопільський державний медичний університет ім. І.Я.Горбачевського»

Адреса – м. Тернопіль, Майдан Волі, 1

G. S. Kurochkin, State University of Medicine and Pharmacy “Nicolae Testemitanu” Chisinau, Moldova

Head of Department of Family Medicine, PHD, University Professor of Medicine and Pharmacy "Nicolae Testemiţanu"

Boulevard Stefan cel Mare si Sfint 165, Chisinau 2004, Moldova

A. V. Levitsky, State University of Medicine and Pharmacy “Nicolae Testemitanu” Chisinau, Moldova

PhD in Genetic’s Coordinator, Department of Science; Lecturer, Department of Molecular biology and Human genetic’s Senior Researcher, laboratory of Human genetics State University of Medicine and Pharmacy "Nicolae Testemitanu"

Bulevardul Ștefan cel Mare și Sfînt 165, Chișinău 2004, Молдова

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Published

2017-07-14

How to Cite

Sheremet, M. I., Sydorchuk, L. P., Shidlovskyi, V. O., Bedeniuk, A. D., Kurochkin, G. S., & Levitsky, A. V. (2017). Apo-1/fas, ctla-4 and bcl-2 genes polymorphism in patients, operated on nodular thyroid pathology. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (2), 13–20. https://doi.org/10.11603/2414-4533.2017.2.7949

Issue

No. 2 (2017)

Section

ORIGINAL INVESTIGATIONS

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