Dynamic Of Balance Of Antioxidant-Prooxidant Mechanism In Internal Organs Under The Influence Of Blood Harnesses And Limb Reperfusion

Abstract

The aim of the work: clarification of the impact of a two-hour use of blood and reperfusion limb tourniquet on the dynamics of antioxidant-oxidative mechanisms in the liver, lung and kidney.

Materials and Methods. Experiments were performed on 36 non-linear male rats weighing 200–220. In the experimental group during anesthesia tiopepntalo sodium (40 mg·kg^-1) proximal to the left leg superimposed strip bundle «SWAT-T»1 cm to 120 minutes. Animals of the research groups were taken out of the experiment, after 60 minutes, 120 minutes, 24 hours, 7 and 14 days after reperfusion. In the control group animals were injected with anesthesia using an equivalent dose of thiopental sodium tow imposed without stopping blood flow and subsequently taken out of the experiment after 2 hours. For the study were liver, kidney and lungs. In homogenates of these reagents to determine the content of thiobarbituric acid (TBA-active products of lipid peroxidation), catalase activity. The ratio of catalase activity / content of TBA-active products of lipid peroxidation was calculated antioxidant-prooxidant index (API).

Results and Discussion. In controlling the intensity of lipid peroxidation and catalase activity increased from the liver to the kidneys and lungs. After reperfusion of limbs in the test bodies was noted increase in the intensity of lipid peroxidation, as evidenced by the accumulation of TBA-active products which the liver was significantly greater than in the control, 14 days in the lungs – up to 7 days in the kidney – within 24 hours. The activity of catalase in organs was significantly greater than controls in all periods of observation with a maximum of 24 hours. Score was significantly greater in the kidney. The value of API in the kidney and lungs increased to 24 hour after reperfusion of limbs and reduced to 14 days without reaching the level of control. In the liver oscillatory dynamics index was the first increase after 1 hour, reduced – 2 hours and further increase to 14 days. These data indicate continued stress adaptation and kompensatoryon mechanisms aimed at eliminating pathogenic factors caused by the imposition of tow a two-hour ischemia and subsequent reperfusion limbs. The results require further in-depth study, but are now pointing to the danger of imposing two-hour tow from the perspective of systemic effects on the body and require additional prevention and correction.

Reperfusion limbs after a two-hour arterial tourniquet is accompanied by prolonged activation of lipid peroxidation in the liver, kidneys and lungs, shown a statistically significant increase of the content of TBA-active products, which in the liver than the control level to the 14^th day after reperfusion in kidney – 24 hours in the lungs – after 7 days. Strengthening lipid peroxidation comes amid increased activity of catalase in all organs, which reaches a maximum after 24 hours and is not reduced to the level of control after 14 days. Balance Antioxidant-prooxidant processes in the studied organs shifted towards strengthening antioxidant mechanisms, which indicates oxidative stress in the long run postreperfusion.