FEATURES OF THE REPARATIVE PROCESS IN EXPERIMENTAL ANIMALS AFTER THE USE OF DIFFERENT METHODS OF FIXATION OF THE ALLOGRAFT IN LAPAROSCOPIC HERNIOPLASTY
DOI:
https://doi.org/10.11603/2414-4533.2025.2.15401Keywords:
allograft, hernioplasty, reparative processAbstract
The aim of the work: to determine the morphological features of the reparative process in the area of the muscular-aponeurotic defect in experimental animals depending on the method of fixation of the polypropylene allograft.
Materials and Methods. The study was conducted on 10 clinically healthy pigs of the Large White breed weighing 20–25 kg, which underwent laparoscopic hernioplasty using a polypropylene allograft. The animals were divided into three groups according to the mesh fixation method. The study was performed on the basis of the vivarium of the Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine. All manipulations with experimental animals were carried out in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986), Order of the Ministry of Health of Ukraine No. 690 dated September 23, 2009 “On Approval of the Procedure for Conducting Scientific Experiments Involving Animals”. Combined general anesthesia with subsequent postoperative anesthesia was used for surgical intervention. Euthanasia was performed humanely in accordance with the AVMA Guidelines for the Euthanasia of Animals (2020).
Results. Microscopic examination 1 month after hernioplasty with an allograft without fixation revealed that the wound defect was filled with granulation tissue with foci of suppuration and bacterial colonies. 1 month after the allograft fixation with a stapler, the wound defect area was dominated by new connective tissue with small foci of maturing granulation tissue. After the self-adhesive propylene mesh fixation, the wound defect was filled with mature connective tissue. It also provides not only mechanical stability of the graft, but also contributes to the optimal microenvironment for the formation of mature connective tissue without signs of chronic inflammation.
Conclusions. The use of self-adhesive polypropylene mesh in laparoscopic hernioplasty in the experiment provides the most favorable conditions for the reparative process - minimal inflammatory reaction, active formation of mature connective tissue and preservation of muscle structure.
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