THE IMPACT OF SKIN DAMAGE ON DIURESIS AND RENAL FILTRATION FUNCTION IN THE CONDITION OF COMBINED TRAUMA AND THE EFFECTIVENESS OF PRP THERAPY
DOI:
https://doi.org/10.11603/2414-4533.2024.2.14850Keywords:
mechanical skin damage, skeletal trauma, blood loss, diuresis, glomerular filtration rateAbstract
The aim of the work: to determine the effect of skin damage on diuresis and renal filtration function in the condition of combined trauma and to evaluate the effectiveness of PRP therapy in correcting the identified disorders.
Materials and Methods. In experiments on 96 sexually mature white male Wistar line rats weighing 200-220 g under thiopental sodium sedation, skeletal trauma (experimental group 1) and combined trauma, which included skeletal trauma complicated by mechanical skin damage (experimental group 2) were modeled. In the experimental group 3, rats with combined trauma underwent PRP therapy: 0.1 ml platelet-rich allogeneic plasma was injected intradermally to a standard depth at the corners of the skin wound. After 3, 7, 14, 21, and 28 days of injury modeling in rats, the functional state of the kidneys was determined by the water load method: diuresis and glomerular filtration rate were determined.
Results and Discussion. Modeled skeletal trauma, which includes femoral fracture and acute blood loss, causes a slowdown in diuresis and glomerular filtration rate. Additional skin damage contributes to the intensification of the disorders. The indicators become statistically significantly lower compared to rats only with skeletal injury. The use of PRP therapy in a group of rats with combined trauma causes a gradual increase in diuresis and glomerular filtration rate from 7 to 28 days of the experiment. Compared to the injured rats without correction, the parameters become statistically significantly higher in terms of diuresis starting from 21 days of the experiment, and in terms of glomerular filtration rate - after 28 days. Thus, on the background of PRP therapy in the period of late manifestations of traumatic disease, there is an improvement in renal filtration function, which indicates the prospects of the method for reducing systemic pathogenic effects on the body and requires further in-depth study.
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