SEX CHARACTERISTICS OF THE DEVELOPMENT OF OXIDATIVE STRESS IN THE HEART OF RATS WITH METABOLICALLY ASSOCIATED STEATOTIC LIVER DISEASE AND ITS CORRECTION WITH INOSITOL

Authors

DOI:

https://doi.org/10.11603/2414-4533.2024.2.14848

Keywords:

oxidative stress, heart, metabolically associated steatotic liver disease, lipid peroxidation, antioxidant system, morphological changes, rats, sex

Abstract

The aim of the work: to study the features of changes in the products of lipid peroxidation and the antioxidant system and morphological changes of heart in rats of different sexes with metabolically associated steatotic liver disease (MASLD) and during correction of the pathological process with inositol.

Materials and Methods. Experiments were performed on 96 rats of different sexes. The animals were divided into 9 groups - control, myo-inositol, D-chiro-inositol, MASLD 4 months, MASLD 4 months + myo-inositol, MASLD 4 months + D-chiro-inositol. In group MASLD 4 (group 2), animals consumed a 20% fructose solution instead of water for 4 months. 2 months after the start of the experiment, they were intragastrically injected with myo-inositol (400 mg/kg) or D-chiro-inositol (30 mg/kg), respectively, in groups 3 and 4. A heart was taken for morphological and biochemical studies; in the heart homogenate, the content of diene and triene conjugates, Schiff's bases, TBA-active products, superoxide dismutase and catalase activity was determined.

Results and Discussion. In the hearts of the control group of male rats, a greater activity of lipid peroxidation processes was noted, compared to females. Metabolically associated steatotic liver disease causes activation of lipid peroxidation processes, with primary lipid peroxidation products and antioxidant activity increasing to a greater extent in males, and secondary and end products of lipid peroxidation increasing in females. Inositols reduce oxidative stress in animals of both sexes. The morphological picture of heart preparations confirmed the obtained biochemical changes.

References

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Published

2024-06-28

How to Cite

LEVCHUK, O. O., & DENEFIL, O. V. (2024). SEX CHARACTERISTICS OF THE DEVELOPMENT OF OXIDATIVE STRESS IN THE HEART OF RATS WITH METABOLICALLY ASSOCIATED STEATOTIC LIVER DISEASE AND ITS CORRECTION WITH INOSITOL. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (2), 56–63. https://doi.org/10.11603/2414-4533.2024.2.14848

Issue

No. 2 (2024)

Section

EXPERIMENTAL INVESTIGATIONS

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