Morphometric analysis of tissues of a muscle-aponeurotic defect in an experiment, under the conditions of hernioplasty using stem cells at different terms of the study
DOI:
https://doi.org/10.11603/2414-4533.2023.4.14344Keywords:
muscle-aponeurotic defect, hernioplasty, morphometric analysis, mesenchymal stem cellsAbstract
The aim of the work: to conduct an analysis of morphometric changes in the area of the muscle-aponeurotic defect of the anterior abdominal wall in an experiment using stem cells.
Materials and Methods. The experimental study was carried out on 76 white sexually mature rats of both sexes, with an initial weight of (215±12) g. Modeling of a muscle-aponeurotic defect by a surgical method was carried out in sterile conditions using the general method of anesthesia. All rats of groups 1 and 3 were modeled with a musculo-aponeurotic defect of the anterior abdominal wall by an acute method up to 2.5 cm in diameter, followed by layer-by-layer suturing of the defect with a single-row continuous wrapping suture with “Vicryl” 3/0 suture material with a barbed needle. In addition, group 3 underwent stem cell injections. In groups 2, 4, the formation of a muscle-aponeurotic defect of the anterolateral abdominal wall 2.5 cm in diameter was also performed, followed by plastic surgery using the “on lay” method with a polypropylene mesh. In group 4, stem cell injections were additionally performed. Animals were removed from the experiment by overdose of thiopental anesthesia for 10 and 30 days of observation. After that, a full-layer sampling of the tissues of the anterior abdominal wall was performed at the place of plastic modeling of the muscle-aponeurotic defect for the next histological examination.
Results and Discussion. In the first two groups, a pronounced inflammatory reaction remained on the 10th day and was weakly expressed on the 30th day, which contributed to the slowing down of the formation of mature connective tissue. A clear positive dynamic was noted in the third group of animals, where a complete subsidence of the inflammatory process and partial formation of a full-fledged scar tissue was found. The best results were obtained in the fourth group of animals using a combination of surgical mesh and stem cells. A complete reduction of the inflammatory reaction and the formation of scar tissue was established.
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