Indicators dynamics of cytolysis and endogenous intoxication under conditions of skeletal trauma complicated by acute blood loss of various degrees and their correction
DOI:
https://doi.org/10.11603/2414-4533.2023.3.14151Keywords:
skeletal trauma, acute blood loss, cytolysis, endotoxicosis, armadineAbstract
The aim of the work: to find out the dynamics of cytolysis indicators and endogenous intoxication under the conditions of skeletal trauma complicated by acute blood loss of various degrees, and to evaluate the effectiveness of detected violation correction with 2-ethyl-6-methyl-3-hydroxypyridine succinate.
Materials and Methods. In experiments on sexually mature white male Wistar line rats, skeletal trauma was simulated under conditions of anesthesia in the first experimental group. In the second and third experimental groups, acute blood loss was additionally induced in the amount of 1 % and 2 % of body weight. In the fourth experimental group, 2-ethyl-6-methyl-3-hydroxypyridine succinate was injected intraperitoneally daily at a dose of 100 mg·kg-1 to rats with skeletal trauma and acute blood loss in the amount of 2 % of body weight for the correction purpose. Examined rats were only injected with thiopental sodium anesthesia. From the experiments, rats of experimental groups 1, 2, and 3 were taken out after 1, 3, and 7 days of the post-traumatic period, rats of experimental group 4 – after 7 days. Blood serum was taken for research, in which markers of cytolysis were determined: the activity of alanine and aspartate aminotransferases (ALT, AST), as well as the content of endogenous intoxication products: fractions of middle mass molecules determined at 254 and 280 nm (МMМ254, MMM280).
Results and Discussion. The infliction of only a skeletal trauma compared to the control group is accompanied by strengthening of cytolysis processes and endogenous intoxication, evidence of which is an increase in ALT and AST activity in blood serum and the accumulation of MMM 254 and MMM 280 fractions in the blood, which are statistically significant only after 3 days of the experiment. Under the conditions of acute blood loss in the volume of 1 % of body weight, the activity of cytolytic enzymes in the blood and the content of the studied fractions of MMM gradually increased from 1 to 3 days and remained at the same level until 7 days. Complication of a skeletal trauma with acute blood loss in the amount of 2 % of body weight causes the greatest violations of the investigated parameters already from the 1st day of the experiment. The indicators gradually increase from 1 to 7 days and in all these terms of the post-traumatic period are statistically significantly higher than in other experimental groups. Intraperitoneal injection of 2-ethyl-6-methyl-3-hydroxypyridine succinate at a dose of 100 mg·kg-1 is accompanied by a significant decrease in the activity of cytolytic enzymes and studied fractions of MMM in blood serum. Although the studied indicators under the influence of the medication don’t reach the level of the control group, it can be argued that 2-ethyl-6-methyl-3-hydroxypyridine succinate belongs to perspective means of pathogenetic correction in complex intensive therapy of traumatic disease and acute blood loss.
Conclusion. Complication of skeletal trauma with acute blood loss in the volume of 1 % of body weight compared to control causes strengthening of cytolysis processes and endogenous intoxication with a maximum after 3 days of the post-traumatic period. Modeling of skeletal trauma and acute blood loss in a volume of 2 % of body weight is accompanied by a gradual increase of ALT and AST activity and the content of MMM 254 and MMM 280 fractions in blood serum from 1 to 7 days of the post-traumatic period, the values of which are statistically greater at all times of the post-traumatic period, compared with traumatized rats with less blood loss. The usage of 2-ethyl-6-methyl-3-hydroxypyridine succinate for correction at a dose of 100 mg·kg-1 in this group reduces the manifestations of cytolysis and endotoxicosis compared to rats with a similar trauma without correction.
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