Dynamics of morphological changes in the endometrioid cyst wall after sclerotherapy with 96 % ethanol
DOI:
https://doi.org/10.11603/2414-4533.2022.4.13605Keywords:
ethanol, pregravid therapy, sclerotherapy, antimuller hormone, endometrioid cyst; ovary, ovulatory reserve, genital endometriosis, stimulation of ovulation, histological examination, laparoscopy, assisted reproductive technologiesAbstract
The aim of the work: to conduct a histological examination of the wall of the endometrioid cyst after sclerotherapy with 96 % ethanol after 1, 3, 5 minutes and establish its structural changes.
Material and Methods. The obtained material after surgical interventions (laparotomy cystectomies) and sclerotherapy with the help of 96 % ethanol with controlled exposure to alcohol in relation to time (1 min, 3 min, 5 min) was fixed in 10 % neutral buffered formalin according to the generally accepted technique. Tissue processing was carried out in a closed vacuum histoprocessor Logos ONE. The finished material was poured into paraffin blocks. Histological sections were prepared on an Amos AMR-400 rotary microtome with a thickness of 4-5 μm (at least two sections for each histological slide), stained with hematoxylin and eosin. Research and photo-registration of the preparations was carried out using an Eclipse Ci-E microscope (Japan) with a Sigeta M3CMOS 14000 digital camera at various magnifications x100, x200, x400.
Results and Discussion. The destructive local effect of 96 % ethanol on the epithelial component of the ovarian endometrioid cyst was studied. Applying different exposure periods, the depth of damage to the epithelial layer, basement membrane, and surrounding cytogenic stroma around the gland was studied. The use of this method in the treatment of cysts will allow a sparing effect on the tissue of the endometrioid cyst of the cortical layer of the ovary with maximum preservation of the surrounding tissue, preserving dormant follicles. By applying different periods of exposure to 96 % ethanol, we reduce tissue trauma, which is combined with acute blood circulation disorders and subsequent scarring.
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