Dynamics of morphological changes in the endometrioid cyst wall after sclerotherapy with 96 % ethanol
DOI:
https://doi.org/10.11603/2414-4533.2022.4.13605Keywords:
ethanol, pregravid therapy, sclerotherapy, antimuller hormone, endometrioid cyst; ovary, ovulatory reserve, genital endometriosis, stimulation of ovulation, histological examination, laparoscopy, assisted reproductive technologiesAbstract
The aim of the work: to conduct a histological examination of the wall of the endometrioid cyst after sclerotherapy with 96 % ethanol after 1, 3, 5 minutes and establish its structural changes.
Material and Methods. The obtained material after surgical interventions (laparotomy cystectomies) and sclerotherapy with the help of 96 % ethanol with controlled exposure to alcohol in relation to time (1 min, 3 min, 5 min) was fixed in 10 % neutral buffered formalin according to the generally accepted technique. Tissue processing was carried out in a closed vacuum histoprocessor Logos ONE. The finished material was poured into paraffin blocks. Histological sections were prepared on an Amos AMR-400 rotary microtome with a thickness of 4-5 μm (at least two sections for each histological slide), stained with hematoxylin and eosin. Research and photo-registration of the preparations was carried out using an Eclipse Ci-E microscope (Japan) with a Sigeta M3CMOS 14000 digital camera at various magnifications x100, x200, x400.
Results and Discussion. The destructive local effect of 96 % ethanol on the epithelial component of the ovarian endometrioid cyst was studied. Applying different exposure periods, the depth of damage to the epithelial layer, basement membrane, and surrounding cytogenic stroma around the gland was studied. The use of this method in the treatment of cysts will allow a sparing effect on the tissue of the endometrioid cyst of the cortical layer of the ovary with maximum preservation of the surrounding tissue, preserving dormant follicles. By applying different periods of exposure to 96 % ethanol, we reduce tissue trauma, which is combined with acute blood circulation disorders and subsequent scarring.
References
Mehedintu, C., Plotogea, M.N., Ionescu, S., & Antonovici, M. (2014). Endometriosis still a challenge. J. Med. Life, 7(3), 349-357. Epub 2014 Sep 25. PMID: 25408753; PMCID: PMC4233437.
D'Alterio, M.N., D'Ancona, G., Raslan, M., Tinelli, R., Daniilidis, A., & Angioni, S. (2021). Management Challenges of Deep Infiltrating Endometriosis. Int. J. Fertil. Steril., 15(2), 88-94. DOI: 10.22074/IJFS.2020.134689. Epub 2021 Mar 11. PMID: 33687160; PMCID: PMC8052801.
Lantsberg, D., Fernando, S., Cohen, Y., & Rombauts, L. (2020). The Role of Fertility Preservation in Women with Endometriosis: A Systematic Review. J. Minim. Invasive Gynecol., 27(2), 362-372. DOI: 10.1016/j.jmig.2019.09.780. Epub 2019 Sep 20. PMID: 31546067.
Tanbo, T, & Fedorcsak, P. (2017). Endometriosis-associated infertility: aspects of pathophysiological mechanisms and treatment options. Acta Obstet. Gynecol. Scand., 96(6), 659-667. DOI: 10.1111/aogs.13082. Epub 2017 Jan 30. PMID: 27998009.
Park, H.J., Kim, H., Lee, G.H., Yoon, T.K., & Lee, W.S. (2019). Could surgical management improve the IVF outcomes in infertile women with endometrioma?: a review. Obstet. Gynecol. Sci., 62(1), 1-10. DOI: 10.5468/ogs.2019.62.1.1. Epub 2018 Dec 27. PMID: 30671388; PMCID: PMC6333762.
Mircea, O., Bartha, E., Gheorghe, M., Irimia, T., Vlădăreanu, R., & Puşcaşiu, L. (2016). Ovarian Damage after Laparoscopic Cystectomy for Endometrioma. Chirurgia (Bucur)., 111(1), 54-57. PMID: 26988540.
Kiesel, L., & Sourouni, M. (2019). Diagnosis of endometriosis in the 21st century. Climacteric., 22(3). 296-302. DOI: 10.1080/13697137.2019.1578743. Epub 2019 Mar 25. PMID: 30905186.
Lee, S.Y., Kim, M.L., Seong, S.J., Bae, J.W., & Cho, Y.J. (2017). Recurrence of Ovarian Endometrioma in Adolescents after Conservative, Laparoscopic Cyst Enucleation. J. Pediatr. Adolesc. Gynecol. 30(2), 228-233. DOI: 10.1016/j.jpag.2015.11.001. Epub 2015 Dec 2. PMID: 26612115.
Moreno-Sepulveda, J., Romeral, C., Niño, G., & Pérez-Benavente, A. (2022). The Effect of Laparoscopic Endometrioma Surgery on Anti-Müllerian Hormone: A Systematic Review of the Literature and Meta-Analysis. JBRA Assist. Reprod., 26(1), 88-104. DOI: 10.5935/1518-0557.20210060. PMID: 34755503; PMCID: PMC8769171.
Ruiz-Flores, F.J., & Garcia-Velasco, J.A. (2012). Is there a benefit for surgery in endometrioma-associated infertility? Curr. Opin. Obstet. Gynecol., 24(3), 136-140. DOI: 10.1097/GCO.0b013e32835175d9. PMID: 22327738.
Barátová, D, Mekiňová, L, Slabá, K, & Crha, I. (2016). Operace endometriomů ovaria a ovariální rezerva Surgical treatment of endometriomas and ovarian reserve. Ceska Gynekol., 81(3), 182-185. Czech. PMID: 27882760.
Roustan, A., Perrin, J., Debals-Gonthier, M., Paulmyer-Lacroix, O., Agostini, A., & Courbiere, B. (2015). Surgical diminished ovarian reserve after endometrioma cystectomy versus idiopathic DOR: comparison of in vitro fertilization outcome. Hum. Reprod., 30(4), 840-847. DOI: 10.1093/humrep/dev029. Epub 2015 Mar 3. PMID: 25740883.
Dunselman, G.A., Vermeulen, N., Becker, C., Calhaz-Jorge, C., D'Hooghe, T., De Bie, B., Heikinheimo, O., … Nelen, W. (2014). ESHRE guideline: management of women with endometriosis. Hum. Reprod., 29(3), 400-412. DOI: 10.1093/humrep/det457. Epub 2014 Jan 15. PMID: 24435778.
Benagiano, G., Petraglia, F., Gordts, S., & Brosens, I. (2016). A new approach to the management of ovarian endometrioma to prevent tissue damage and recurrence. Reprod. Biomed. Online., 32(6), 556-562. DOI: 10.1016/j.rbmo.2016.03.001. Epub 2016 Mar 11. PMID: 27037156.
Papaleo, E, Ottolina, J, Viganò, P, Brigante, C, Marsiglio, E, De Michele, F, & Candiani, M. (2011). Deep pelvic endometriosis negatively affects ovarian reserve and the number of oocytes retrieved for in vitro fertilization. Acta Obstet. Gynecol. Scand., 90(8), 878-884. DOI: 10.1111/j.1600-0412.2011.01161.x. Epub 2011 Jun 14. PMID: 21542809.
Sanchez, A.M., Vanni, V.S., Bartiromo, L., Papaleo, E., Zilberberg, E., Candiani, M., Orvieto, R., & Viganò, P. (2017). Is the oocyte quality affected by endometriosis? A review of the literature. J. Ovarian. Res., 10(1), 43. DOI: 10.1186/s13048-017-0341-4. PMID: 28701212; PMCID: PMC5508680.
Simopoulou, M., Rapani, A., Grigoriadis, S., Pantou, A., Tsioulou, P., Maziotis, E., Tzanakaki, D., … Vlahos, N. (2021). Getting to Know Endometriosis-Related Infertility Better: A Review on How Endometriosis Affects Oocyte Quality and Embryo Development. Biomedicines, 9(3), 273. DOI: 10.3390/biomedicines9030273. PMID: 33803376; PMCID: PMC7998986.
Ban Frangež, H., Vrtacnik Bokal, E., Štimpfel, M., Divjak Budihna, T., Gulino, F.A., Garzon, S., … Laganà, A.S. (2022). Reproductive outcomes after laparoscopic surgery in infertile women affected by ovarian endometriomas, with or without in vitro fertilisation: results from the SAFE (surgery and ART for endometriomas) trial. J. Obstet. Gynaecol., 42(5), 1293-1300. DOI: 10.1080/01443615.2021.1959536. Epub 2021 Sep 29. PMID: 34585638.
Kitajima, M., Defrère, S., Dolmans, M.M., Colette, S., Squifflet, J., Van Langendonckt, A., & Donnez, J. (2011). Endometriomas as a possible cause of reduced ovarian reserve in women with endometriosis. Fertil. Steril., 96(3), 685-691. DOI: 10.1016/j.fertnstert.2011.06.064. Epub 2011 Jul 29. PMID: 21802672.
Cohen, A., Almog, B., & Tulandi, T. (2017). Sclerotherapy in the management of ovarian endometrioma: systematic review and meta-analysis. Fertil Steril., 108(1), 117-124.e5. DOI: 10.1016/j.fertnstert.2017.05.015. Epub 2017 Jun 1. PMID: 28579409.
Akamatsu, N., Hirai, T., Masaoka, H., Sekiba, K., & Fujita, T. (1988). Ultrasonically guided puncture of endometrial cysts--aspiration of contents and infusion of ethanol. Nihon Sanka Fujinka Gakkai Zasshi., 40(2), 187-191. Japanese. PMID: 3283269.
Bahriy, M.M., Dibrova, V.A., Popadynets, O.H., & Hryshchuk, I.M. (2016). Metodyky morfolohichnykh doslidzhen: monohrafiya [Methods of morphological research: monograph]. Vinnytsia: Nova knyha [in Ukrainian].
André, G.M., Vilarino, F.L,, Christofolini, D.M., Bianco, B., & Barbosa, C.P. (2011). Aspiration and ethanol sclerotherapy to treat recurrent ovarian endometriomas prior to in vitro fertilization – a pilot study. Einstein (Sao Paulo), 9(4), 494-498. English, Portuguese. DOI: 10.1590/S1679-45082011AO2081. PMID: 26761252.
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