Determining the prevalence of acute peritonitis in an experiment

Abstract

The aim of the work: to study of the possibilities of objective assessment of the prevalence of AP in an experiment.

Material and Methods. 50 white non-linear rats. Acute peritonitis (AP) was modeled by intraperitoneal injection of a 20 % autofecal mixture. In 10 healthy (control) and 40 animals with HP models, 6, 12, 24, 48 hours after its initiation, a laparotomy was performed and the width of the scattering zone of laser beams (WSZLB) with wavelengths λ=0.63 μm and λ=0 was determined. 5 μm parietal peritoneum, after which it was taken for histological examination.

Results and Discussion. In the control of WSZLB at a wavelength of λ=0.63 μm = 6.2±0.12, at a wavelength of λ=0.5 μm – 6.0±0.15. After 6 hours from the time of modeling, signs of serous AP were detected, and later - purulent AP. After 6 hours, the WSZLB at a wavelength of λ=0.63 μm = 11.6±0.17, at a wavelength of λ=0.5 μm – 6.7±0.12, after 12 hours – 13.95±0, 16 and 7.62±0.12, after 24 hours – 16.17±0.16 and 9.52±0.12, after 24 hours – 16.17±0.16 and 9.52±0.12, after 48 hours – 17.15±0.14 and 10.27±0.12. The ratio of WSZLB indicators λ=0.63 μm / λ=0.5 μm in the control = 1.06±0.03, after 6 hours – 1.76±0.04, after 12 hours – 1.85±0.04 , after 24 hours – 1.71±0.02, after 48 hours – 1.68±0.02, which constantly significantly exceeded the control indicators (p<0.01).

In the case of AP, the parietal peritoneum with wavelengths of λ=0.63 μm and λ=0.5 μm increases statistically significantly, but the absolute parameters of the indicator are characterized by individual variability. The ratio of the parameters of WSZLB with a wavelength of λ=0.63 μm to that with a wavelength of λ=0.5 μm is statistically significantly different in unchanged and inflamed areas of the parietal peritoneum. Indicators of the ratio of less than 1.5 indicate the absence of inflammation, and an increase in the indicators indicates an inflammatory process.