Morpho-structural changes of diaphragm after simulated increased intra-abdominal pressure of different duration

Abstract

The aim of the work: to assess the degree of morpho-structural changes of the muscular-aponeurotic elements of the diaphragm under the action of experimentally simulated increased intra-abdominal pressure of different duration.

Materials and Methods. The work was performed on 12 nonlinear, mature white rats, both sexes, weighing 180–200 g. The experimental animals were divided into 4 groups depending on the duration of intense intra-abdominal pressure: group 1 (3 animals) – up to 1 hour, group 2 (3 animals) – up to 3 hours, group 3 (3 animals) – up to 5 hours and group 4 (3 animals) – more than 5 hours. Experimental elevated pressure was simulated using a cuff and tonometer. In order to assess the structural changes in the experimental white rats after their withdrawal from the experiment, the diaphragm was dissected and subjected to macroscopic examination. The next stage was the collection of tissue from different anatomical departments (dome, legs, tendon center) with their subsequent histological examination.

Results and Discussion. The results of histological examination indicate that general anesthesia with increased intra-abdominal pressure affects the condition of organs and tissues of white rats and, in particular, the condition of the diaphragm. Morphologically, these changes are characterized by the development of hemodynamic disorders, the phenomena of disorganization of the fibrous structures of connective tissue, dystrophic changes in muscle tissue, the emergence of an inflammatory reaction. Evaluating the intensity of these changes in experimental groups with different duration of general anesthesia, we can trace a direct relationship between the severity of structural changes and the duration of action of the studied factor on the body of experimental animals.