SCREENING INVESTIGATIONS OF PHYTOCOMPOSITION ON THE BASIS OF DRY EXTRACT OF GOAT'S-RUE, BLUEBERRY AND TAURINE WITH POTENTIAL HYPOGLICEMIC PROPERTIES

Abstract

The aim of the work. Screening studies of phytocompositions on the basis of dry extract of goat's-rue, blueberry and taurine with potential hypoglycemic properties.

Materials and Methods. The object of the docking is combine phytocomposition on the basis of dry extract of goat's-rue, blueberry and taurine, as well as its liposomal form with the conventional name "Halevit". The research was carried out to determine the most effective for the hypoglycemic activity of doses of phytocomposition on the basis of the goat's-rue and its liposomal form "Halevit" using an oral test of glucose tolerance in animals. The hypoactivity of the investigated extracts was used to decrease the level of glucose in the the maximum development hyperglycemic for glaciation of glucose paths in the presence of a glucose curve. Concentration of glucose in the blood was noted for a full-blown, "Accu-Chek performa" glucose test.

Results and Discussion. Screening assay of hypoglycemic activity of goat's-rue phytocomposition and its liposomal form under the conventional name «Halevit» have shown the ability of phytopreparations at doses of 50 mg/kg, 75 mg/kg, 100 mg/kg, 125 mg/kg and 150 mg/kg to decrease the level of glycemia at oral glucose tolerance test in intact white rats. It has been established that the most pronounced hypoglycemic activity of phytocompositions was found at doses of 100, 125 and 150 mg/kg and in the period of 30-60 minutes after the simulation of test. The decrease in the level of glycemia under the influence of the investigated phytocompositions in that period of the experiment was maximal and acquired statistically important differences from the values of animals that did not have phytocorrection or had it at lower doses of 50 mg/kg and 75 mg/kg. It has been found that further increase of the dose of phytocompositions up to 125 mg/kg and 150 mg/kg did not lead to statistically significant additional increase in hypoglycemic effect of phytocompositions at oral glucose tolerance test.

Conclusions. The most optimal dose, which provides the highest hypoglycemic activity of phytocompositions on the dry extract of goat's-rue, blueberry and taurine in the oral glucose tolerance test, is a dose of 100 mg/kg. The most pronounced hypoglycemic effect was manifested when introduced into animals by phytocomposition "Halevit".