ON THE L-TRYPTOPHAN AND THIOTRIAZOLINE QUANTITATION IN MODEL MIXTURE

Authors

  • L. I. Kucherenko Zaporizhzhia State Medical University
  • І. А. Мazur Zaporizhzhia State Medical University
  • S. O. Borsuk Zaporizhzhia State Medical University
  • O. O. Portna Zaporizhzhia State Medical University

DOI:

https://doi.org/10.11603/2312-0967.2018.2.9002

Keywords:

L-tryptophan, thiotriazoline, fixed combination, model mixture, high-performance liquid chromatography.

Abstract

The aim of work. To develop standardization methods the model mixture, which consists of L-tryptophan and thiotriazoline in the therapeutic ratio 4:1.

Materials and Methods. During the study, 6 series of the model mixture of L-tryptophan and thiotriazoline in a ratio of 4:1 (the active ingredient content per tablet 200 mg and 50 mg respectively) were prepared in the laboratory and was developed the standardization method for their simultaneous determination by HPLC.

Results and Discussion. An analysis of 6 series of a model mixture (in each of 6 experiments) was carried out according to the developed HPLC method. As a result of the study, we have determined the content of L-tryptophan, which is from 197.2 mg to 204.9 mg, thiotriazoline from 48.9 mg to 50.5 mg, that meets the requirements of the current normative documentation.

Results. In the course of work, we have developed a high-sensitivity, reproducible, reliable, high-precision method of simultaneous standardization of active substances, which is planned to be used for the stage-by-stage control of the quality of L-tryptophan and thiotriazoline tablets.

Author Biographies

L. I. Kucherenko, Zaporizhzhia State Medical University

Professor, Doctor of Pharmacy, Zaporizhzhia State Medical University, Head of the Department of Pharmaceutical Chemistry, Vice-President of SPA "Farmatron”

І. А. Мazur, Zaporizhzhia State Medical University

Professor, Doctor of Pharmacy, Zaporizhzhia State Medical University, Professor of the Department of Pharmaceutical Chemistry, President of SPA "Farmatron”

S. O. Borsuk, Zaporizhzhia State Medical University

Assistant professor of the Department of Pharmaceutical Chemistry, Zaporizhzhia State Medical University

O. O. Portna, Zaporizhzhia State Medical University

M. Sc. Pharm., Associate Professor, Department of Pharmaceutical Chemistry, Zaporizhzhia State Medical University, SPA "Farmatron”

References

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Published

2018-06-21

How to Cite

Kucherenko, L. I., Мazur І. А., Borsuk, S. O., & Portna, O. O. (2018). ON THE L-TRYPTOPHAN AND THIOTRIAZOLINE QUANTITATION IN MODEL MIXTURE. Pharmaceutical Review Farmacevtičnij časopis, (2), 54–58. https://doi.org/10.11603/2312-0967.2018.2.9002

Issue

No. 2 (2018)

Section

Analysis of drugs

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