ACUTE TOXICITY STUDY OF ARGOCIDE DRUG WHEN ADMINISTERED INTRAPERITONEALLY
DOI:
https://doi.org/10.11603/2312-0967.2018.1.8694Keywords:
preclinical research, veterinary drug, intraperitoneal administration, acute toxicity, Argocide.Abstract
The aim of the work. Study of acute toxicity of the veterinary drug Argocide –solution for intracisternal administration with intraperitoneal administration to sexually mature white rats.
Materials and Methods. The object of the study was a series of a combined drug of Argocide containing silver citrate, D-panthenol and L-arginine for the treatment of subclinical mastitis in cattle. The acute toxicity study was carried out in experiments on white non-linear sexually mature rats of both sexes, according to the requirements for potential drugs. The tests were performed with intraperitoneal administration of the drug in various doses – from 2.0 to 6.3 ml / kg. Statistical processing of data was performed using the t-test of the Student. For the statistical processing of data, the MS Excel program was used.
Results and Discussion. As a result of the study of acute toxicity of Argoсide, it was established that integral indices of animal life– weight and body temperature did not undergo significant individual changes during 14 days of observation after single intraperitoneal administration. In studies of doses ranging from 1.58 ml / kg body weight of the animal to 5.0 ml / kg, it is determined that no case of death of domestic animals has been recorded with intraperitoneal administration of the drug at any dose. When using the drug in doses of 2.5 ml / kg and above, the animals showed clinical signs of intoxication immediately after the administration of the drug, which may be due to irritation of the peritoneum. Three days after the intraperitoneal administration of Argocide, no clinical signs of intoxication were observed.
Conclusions. The analysis of the obtained data allows to classify the Argocide drug, the solution for intracisternal injection according to the degree of safety to relatively harmless preparations (VI class), since the average lethal dose exceeds 3.0 ml / kg of body weight of the animal with intraperitoneal administration.References
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