SPECTROPHOTOMETRIC DETERMINATION OF ATENOLOL IN DOSAGE FORMS USING 2,3-DICHLORO-1,4-NAPHTOQUINONE
DOI:
https://doi.org/10.11603/2312-0967.2017.4.8397Keywords:
atenolol, 2, 3-dichloro-1, 4-napthoquinone, spectrophotometry, assay, validation studies.Abstract
The aim of the work. Developing and validating the spectrophotometric method for atenolol assay using absorption spectrophotometry.
Materials and Methods. Atenolol working standard, 2,3-dichloro-1,4-naphthoquinone and the sample of finished dosage forms were used.
Results and Discussion. It was experimentally established that atenolol reacts with 2,3-dichloro-1,4-naphthoquinone in DMF medium to formation the colored reaction product with absorption maximum at 493 nm. The proposed method was subjected to validation tests. The method was validated for the parameters like linearity, precision, accuracy, robustness and scope of application. Beer’s law was obeyed over the concentration range of 11.20–19.60 mg/100 ml with correlation coeffіcient 0.9990. The linearity ranges were calculated with the help of regression analysis by means of least squares.
Conclusion. The spectrophotometric method of atenolol assay was developed and validated. This procedure is successfully applied for dosage forms analysis. Investigation results show that the procedure is precise, simple and relevant to be applied at the quality control laboratories for dosage forms.References
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