STUDY OF HEPATOPROTECTIVE ACTIVITY OF LIQUID EXTRACT OF SUMMER SAVORY HERB UNDER CONDITION OF TETRACHLOROMETHANE HEPATITIS
DOI:
https://doi.org/10.11603/2312-0967.2017.2.7899Keywords:
tetrachloromethane hepatitis, liquid extract, Satureja hortensis.Abstract
The aim of the work. It was carried out the investigation of the hepatoprotective activity of liquid extract of Summer Savory (Satureja hortensis L.) herb on a model of acute liver injury caused by carbon tetrachloride.
Materials and Methods. The liquid extract was prepared by extracting the herb with ethanol (70 %, 50 % and 30 %), followed by combining and concentrating these extracts; its dosage was carried out according to the dry residue. It was twice administered the 50 % tetrachloromethane solution at a dose of 1 ml/kg of body weight of rats for induction of hepatitis. Doses of 50 mg/kg, 100 mg/kg and 200 mg/kg of the liquid extract were chosen for studying of their hepatoprotective activity; «Silibor» was used as a reference drug in a dose of 50 mg/kg.
Results and Discussion.Using of the liquid extract of Summer Savory herb with preventive maintenance against the tetrachloromethane hepatitis in rats signifi cantly reduced the general manifestations of intoxication. All rats after receiving the extract have survived, and it can indicate the prospect of hepatoprotective activity of investigated extract. The most signifi cant changes of biochemical parameters set by using the extract at a dose of 200 mg/kg. Investigated extract at dose 200 mg/kg did not signifi cantly differ from the reference drug according to infi uence on the content of malonic dialdehyde, molecules of average weight and the coefficient of liver mass. It prevailed over the «Silibor» by such biochemical parameters as the content of total protein and superoxide dismutase. Using of 50 mg kg and 100 mg/kg doses did not always have a normalizing effect on data of affected animals.Conclusions. This study determined the hepatoprotective effect of Summer Savory liquid extract at the dose of 200 mg/kg after administration to tetrachloromethane-affected rats.
References
Baraboy VA. Plant phenols and human health. [Растительные фенолы и здоровье человека] Moscow: Nauka; 1984. Russian.
Galuzinska LV. [Experimental study of hepatoprotective activity of the polyphenolic extract of aerial parts lyadventsyu horned]. Ukr. biofarm. zhurnal. 2013;1(24):28-33. Ukrainian.
Gromashevskaya LL. ["Average molecules” as one of the factor of body metabolic intoxication]. Lab. diagnostika. 1997;1:11-6. Russian.
Gubskiy Yul. Correction of liver chemical demage. [Корекция химического поражения печени]. K.: Zdorovya, 1989. Russian.
[Derzhavna Farmakopeia Ukrainy] / Derzhavne pidpryiemstvo «Naukovo-ekspertnyi farmakopeinyi tsentr». 1-e vyd. Dopovnennia 1. Kharkiv: RIREG; 2004. Ukrainian.
[Doklinichni doslidzhennia likarskykh zasobiv (metodychni rekomendatsii)] Za red. chlen-kor. AMN Ukrainy OV. Stefanova. Kyiv: Avicenna; 2001. Ukrainian.
Zarivna NO, Vronska LV. [Standartization of liquid extract of savory herb]. Ukr. biofarm. zhurnal. 2012;5-6:108-12. Ukrainian.
Medicinal herbs : encyclopedic guide. [Лікарські рослини: енциклопедичний словник] Vidp. red. A.M. Hrodzynskyi. K.: Vyd-vo "Ukrainska entsyklopediia" im. M.P. Bazhana; 1992. Ukrainian.
Martyshuk TV. [Influence of oxydative stress on the system of antioxidant defence of rats’ body]. Visnyk Dniprop. un-tu. Biolohiia, medytsyna. 2016;7(1):8-12. Ukrainian.
Oleshchuk OM, Ivankiv Yal, Shumska OA. [Study of hepatoprotective melatonin properties and the role of polymorphism of MTNR1B gene in patients with diabetes mellitus type 2]. Medychna ta klinichna khimiia. 2016;18 (4):50-4. Ukrainian.
Pentyuk AA, Moroz LV, Palamarchuk OV. [Liver lesion by xenobiotics]. Sovremennye problemy toksikologii. 2001;2:8-16. Russian.
Pyda VP. [Use of dense extract of buckthorn in the condition of chronic combined hepatitis in rats]. Medychna khimiia. 2011;13(1):78-81. Ukrainian.
Popovych VP, Hromovyk BP, Siatynia VA. Hepatoprotective potential of herbs : monography. [Гепатопротекторний потенціал рослин : монографія] K.: Interservis; 2012. Ukrainian.
Shanaida MI [Study of qualitative composition and quantitative content of flavanoids in the herb of representatives of Nepetoideae Lamiaceae]. Farm. zhurnal. 2015;4:71-6. Ukrainian.
Shanayda MI, Marchyshyn SM. [Qualitative composition and quantitative content of hydroxycoric acids in aboveground part of cultivated representatives of Lamiaceae family]. Vestnik YUKGFA. 2016;1(74):23-6. Russian.
Shtroblya AL, Fira LS, Lykhaky PG [Selection of minimally active dose of dry extract from leaves of apricot on conventional models of liver injury by tetrachlormethane]. Farmatsevtychnyi chasopys. 2012;3:132-5. Ukrainian.
[European convention for the protection of vertebrate animals used for experimental and other scientific purposes]. Council of Europe: Strasbourg; 1986.
Foti МС. [Antioxidant properties of phenols] J Pharm Pharmacol. 2007; 59(12):1673-85.
Tresina PS, Paulpriya К, Mohan VR [Hepatoprotective activity of Aristolochia bracteata Retz against carbon tetrachloride induced hepatic damage] Int J Pharm Sci Rev Res. 2016;40(1),No.32:164-9.
Khomdram S, Potsangbam К. [Polyphenolic compounds and free radical scavenging activity in eight Lamiaceae herbs of Manipur] Not Sci Biol. 2011;I.3:108-13.
Perez Gutierrez RM, Gallardo Navarro YT [Antioxidant and hepatoprotective effects of the methanol extract of the leaves of Satureja macrostema] Pharmacogn Mag. 2010;6(22):125-31.
Shanaida M, Ivanusa I, Kernychna I. [Phytochemical analysis of secondary metabolites of Satureja hortensis L.] Int J Pharm Pharm Sci. 2017;9(2):315-8.
Hamidpour R, Hamidpour S, Hamidpour M, Shahlari M, Sohraby M. [Summer Savory: from the selection of traditional applications to the novel effect in relief, prevention, and treatment of a number of serious illnesses] J Tradit Compl Med. 2014;4(3):140-4.
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