NEUROPROTECTIVE ACTIVITY OF TABLETS CONTAINING DRY EXTRACT OF PEONY ROOTS, L-TRYPTOPHAN AND GLYCINE IN EXPERIMENTAL CRANIO-CEREBRAL TRAUMA
DOI:
https://doi.org/10.11603/2312-0967.2025.4.15589Keywords:
traumatic brain injury, neuroprotection, dry peony root extract, L-tryptophan, glycineAbstract
The aim of our work was to study the neuroprotective effect of new combination tablets with dry peony root extract, L-tryptophan, and glycine under conditions of experimental traumatic brain injury.
Materials and Methods. Experiments were conducted on sexually mature male rats that were modeled for traumatic brain injury, and tablets with dry peony root extract, L-tryptophan and glycine, and citicoline were used for correction.
Results and Discussion. Tablets containing dry peony root extract, L-tryptophan, and glycine at a dose of 35 mg/kg have a pronounced neuroprotective effect in an experimental model of traumatic brain injury in rats. The use of the drug was accompanied by a significant reduction in neurological deficit, as confirmed by the McGraw and mNSS scales. The animals showed improvement in motor activity, coordination, and exploratory behavior, as evidenced by the results of the «Open Field» and «Rota-rod» tests.
The drug under study effectively reduced the manifestations of oxidative stress in brain tissue. This is confirmed by a statistically significant decrease in the level of nitrotyrosine, a marker of oxidative protein modification. Thus, on the eighth day after traumatic brain injury, the concentration of nitrotyrosine decreased by 56% in the cytosolic fraction and by 55% in the mitochondrial fraction. In addition, an increase in the activity of antioxidant enzymes was observed: superoxide dismutase, catalase, and glutathione peroxidase, which indicates the restoration of the antioxidant potential of brain tissue and an increase in the body's adaptive reserves.
Conclusions. Combined tablets containing dry peony root extract, L-tryptophan, and glycine at a dose of 35 mg/kg have a pronounced neuroprotective effect in an experimental model of traumatic brain injury in rats. The studied agent reduced the manifestations of oxidative stress in brain tissue and increased the adaptive reserves of the body by reducing the cytosolic and mitochondrial fractions of the marker of oxidative modification of proteins, nitrotyrosine.
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