STUDY OF ACUTE TOXICITY OF EXTRACTS FROM WOOD COW-WHEAT (MELAMPYRUM NEMOROSUM L.)
DOI:
https://doi.org/10.11603/2312-0967.2025.2.15392Keywords:
wood cow-wheat, herb, liquid extract, biologically active substances, medicinal plant raw materials, acute toxicity, laboratory animalsAbstract
The aim of the work was to investigate the acute toxicity of liquid extracts developed from the herb of wood cow-wheat (Melampyrum nemorosum L.)
Materials and Methods. The study of acute toxicity of the liquid extracts of the wood cow-wheat herb was conducted according to the methodology of preclinical studies on the safety of medicinal products. The research was carried out on white outbred mice. The condition of each animal was recorded at least once a day. After the experiment, macroscopic examinations and descriptions of the internal organs were conducted. To assess the toxic effects of the wood cow-wheat herb extracts on the mice's bodies, the body weight of animals in all studied groups was determined at the beginning of the experiment and on the 14th day of the experiment.
Results and Discussion. The studied extracts obtained from the wood cow-wheat herb passed the tests for basic numerical indicators and microbiological purity. In studying acute toxicity, it was noted that the studied extracts, when administered intragastrically, do not cause death or intoxication in animals over a period of 14 days. The organs of the animals that were administered the studied preparations did not differ from those of intact animals. The analysis of the mass coefficients of the internal organs confirms the absence of pathological changes in the functional state of the experimental animals compared to the intact ones. The biochemical indicators of the blood of mice under the influence of the extracts of the wood cow-wheat herb are within the range of indicators of the intact group.
Conclusions. 1. The intragastric administration of extracts obtained from the wood cow-wheat at a maximum dose (5000 mg/kg body weight of the animal) did not lead to the death of the animals, and there were no changes in biochemical blood parameters and morphological structure of the internal organs of the test animals, indicating the absence of toxic effects. 2. The results indicate that the median lethal dose (LD50) exceeds the administered dose of 5000 mg/kg to the animals, providing grounds to classify the substances under study as class V toxicity - practically non-toxic (LD50 > 5000 mg/kg).
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