PHYTOCHEMICAL AND MICROMORPHOLOGICAL ANALYSIS OF THE SEEDS COLLECTED FROM THE NEWLY DEVELOPED NIGELLA DAMASCENA L. CULTIVAR
DOI:
https://doi.org/10.11603/2312-0967.2024.3.14867Keywords:
love-in-a-mist, new cultivar, seeds, volatile compounds, total phenolic content, micromorphometryAbstract
The aim of the work. In this study, the total phenolic content (TPC) and composition of volatile compounds in the seeds of the newly developed cultivar 'Rizdviana zirochka' of Nigella damascena L. (love-in-a-mist) were analyzed. The morphometric characteristics of these seeds were also evaluated.
Materials and Methods. The gas chromatography-mass spectrometric method (GC/MS) was applied to analyze volatile compounds' qualitative composition and quantitative content. The Agilent Technologies 6890 chromatograph with a mass spectrometric detector was applied. The quantitative content of volatile components was determined using tridecane as the internal standard. The TPC was estimated using the Folin-Ciocaltey reagent following the Singleton and Rossi method. The morphometric analysis of seeds was conducted using AxioCam MRc5 light microscope and AxioVision Rel. 4.8.2 software.
Results and Discussion. Among the volatile compounds detected in the newly developed cultivar 'Rizdviana zirochka' of Nigella damascena, three terpenoids were found to be dominant: beta-elemene (34.01 %), germacrene A (28.16 %), and damascenine (21.97 %). The TPC of the 80% methanol extract from the studied cultivar seeds was measured to be (29.37±0.59) mg GAE/g. Additionally, the conducted morphometric analysis revealed the key morphological parameters of these seeds.
Conclusions. In this study, the TPC and volatile compounds composition in the seeds of the newly developed 'Rizdviana zirochka' cultivar of Nigella damascena were analyzed, and the morphometric characteristics of these seeds were evaluated.
References
Fierascu RC, Fierascu I, Baroi AM, Ortan A. Selected Aspects Related to Medicinal and Aromatic Plants as Alternative Sources of Bioactive Compounds. Int J Mol Sci. 2021; 22(4):1521. DOI: 10.3390/ijms22041521.
Kisiriko M, Anastasiadi M, Terry LA, Yasri A, Beale MH, Ward JL. Phenolics from Medicinal and Aromatic Plants: Characterisation and Potential as Biostimulants and Bioprotectants. Molecules. 2021; 26(21):6343. DOI: 10.3390/molecules26216343.
Toma CC, Olah NK, Vlase L, Mogoșan C, Mocan A. Comparative Studies on Polyphenolic Composition, Antioxidant and Diuretic Effects of Nigella sativa L. (Black Cumin) and Nigella damascena L. (Lady-in-a-Mist) Seeds. Molecules. 2015; 20(6): 9560-9574. DOI: 10.3390/molecules20069560.
Badalamenti N, Modica A, Bazan G, Marino P, Bruno M. The ethnobotany, phytochemistry, and biological properties of Nigella damascena - A review. Phytochemistry. 2022; 198:113165. DOI: 10.1016/j.phytochem.2022.113165.
Black cumin (Nigella sativa) seeds: Chemistry, Technology, Functionality, and Applications / Ed. By Mohamed Fawzy Ramadan. Springer Nature Switzerland AG 2021. DOI: 10.1007/978-3-030-48798-0.
Salehi B, Quispe C, Imran M, et al. Nigella Plants - Traditional Uses, Bioactive Phytoconstituents, Preclinical and Clinical Studies. Front Pharmacol. 2021;12: 625386.
Dalli M, Bekkouch O, Azizi SE, Azghar A, Gseyra N, Kim J. Nigella sativa L. Phytochemistry and pharmacological activities: A Review. Biomolecules. 2021;12(1): 20. DOI: 10.3390/biom12010020.
Dąbrowski G, Czaplicki S, Konopka I. Variation in the Composition and Quality of Nigella sativa L. Seed Oils-The Underestimated Impact on Possible Health-Promoting Properties. Molecules. 2024; 29(6), 1360. DOI: 10.3390/molecules29061360.
NCT03175757. A Study to Evaluate the Effects of a Turmeric and Black Cumin Seed Formulation on Cholesterol Levels (Location: Fort Lauderdale, Florida, USA).https://clinicaltrials.gov/study/NCT03175757?cond=nigella&term=seeds&page=2&rank=12.
NCT00327054. Effectiveness of Nigella sativa (Kalonji) Seed in Dyslipidemia ((Location: Karachi, Sindh, Pakistan) https://clinicaltrials.gov/search?cond=metabolic&term=nigella.
NCT04401202. Nigella sativa in COVID-19 (Location: Jeddah, Saudi Arabia). https://clinicaltrials.gov/study/NCT04401202?cond=nigella&term=seeds&rank=8.
NCT05693597. Evaluation of the Effect of Nigella sativa for the Prophylaxis to Radiation Induced Dermatitis in Breast Cancer Patients. (Location: Сairo, Egypt). https://clinicaltrials.gov/search?cond=Inflammation&term=nigella.
Scognamiglio M, Maresca V, Basile A, et al. Phytochemical Characterization, Antioxidant, and Anti-Proliferative Activities of Wild and Cultivated Nigella damascena Species Collected in Sicily (Italy). Antioxidants. 2024;13(4):402. DOI: 10.3390/antiox13040402.
Dadandi M, Kökdil G, İlçim A, Özbilgin B. Seed macro and micro morphology of the selected Nigella (Ranunculaceae) taxa from Turkey and their systematic significance. Biologia 2009; 64/2: 261–270. DOI: 10.2478/s11756-009-0030-x.
Nargout-Jzntac D, Kelly MT, Pelissier Y et al. Morphological, microscopic and chemical comparison between Nigella sativa L. cv (black cumin) and Nigella damascena L. cv. Journal of Food, Agriculture and Environment. 2013;11(1):132-138.
Korablova OA, Shanaida MI. Svidotstvo No 230642 pro avtorstvo na sort roslyny «Rizdvyana zirochka» vydu Nigella damascena L. Kyiv. 2023.
Jalali-Heravi M, Parastar H, Sereshti H. Towards obtaining more information from gas chromatography-mass spectrometric data of essential oils: an overview of mean field independent component analysis. J Chromatogr A. 2010; 1217(29): 4850–4861. DOI: 10.1016/j.chroma.2010.05.026.
Hudz N, Yezerska O, Shanaida M, Sedláčková VH, Wieczorek PP. Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts. Pharmacia. 2019; 66(4): 209-215. DOI: 10.3897/pharmacia.66.e38976.
Xie Q, Li F, Fang L, Liu W, Gu C. The Antitumor Efficacy of β-Elemene by Changing Tumor Inflammatory Environment and Tumor Microenvironment. BioMed Research International. 2020; 6892961. DOI: 10.1155/2020/6892961.
Dong XM, Chen L, Wu P, et al. Targeted metabolomics reveals PFKFB3 as a key target for elemene-mediated inhibition of glycolysis in prostate cancer cells. Phytomedicine. 2024;123:155185.
Sieniawska E, Sawicki R, Golus J, et al. Nigella damascena L. Essential Oil-A Valuable Source of β-Elemene for Antimicrobial Testing. Molecules. 2018;23(2):256.
Cheng J, Zuo Y, Liu G, et al. Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene. Bioresour Bioprocess. 2023; 10(1):38.
Halima Rchid, Rachid Nmila, Jean-Marie Bessière Bessière et al. Volatile Components of Nigella damascena L. and Nigella sativa L. Seeds. Journal of Essential Oil Research. 2004;16(6):585-587. DOI: 10.1080/10412905.2004.9698804.
Sieniawska E, Michel P, Mroczek T, Granica S, Skalicka-Woźniak K. Nigella damascena L. essential oil and its main constituents, damascenine and β-elemene modulate inflammatory response of human neutrophils ex vivo. Food Chem Toxicol. 2019;125:161-169. DOI: 10.1016/j.fct.2018.12.057.
Khattak KF, Simpson TJ, Ihasnullah. Effect of gamma irradiation on the extraction yield, total phenolic content and free radical-scavenging activity of Nigella sativa seed. Food Chemistry. 2008;110(4):967-972. DOI: 10.1016/j.foodchem.2008.03.003.
Benazzouz-Smail L, Achat S, Brahmi F, et al. Biological Properties, Phenolic Profile, and Botanical Aspect of Nigella sativa L. and Nigella damascena L. Seeds: A Comparative Study. Molecules. 2023;28(2):571. DOI: 10.3390/molecules28020571.
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