SPECTROPHOTOMETRIC DETERMINATION OF 1-BENZYL-8-MORPHOLINOTEOBROMINE
DOI:
https://doi.org/10.11603/2312-0967.2024.1.14518Keywords:
1-benzyl-8-morpholinoteobromine, quantitative determination, UV spectrophotometry, validation, AGREEAbstract
The aim of the work. Development and validation of a spectrophotometric method for quantitative determination of 1-benzyl-8-morpholinoteobromine in substance according to pharmacopoeial requirements.
Materials and Methods. The research material is the substance of 1-benzyl-8-morpholinotheobromine. The solvent is methanol. Analytical equipment: spectrophotometer Specord 200 (Analytic Jena AG, Germany), electronic laboratory scale RADWAG XA 210.4Y, ultrasonic bath Sonorex Digitec DT100H, class A measuring vessels.
Results and Discussion. A wavelength range of 260–320 nm with a maximum absorbance at 292 nm was selected for the development of the quantitative determination methodology. The validation of the method was carried out in accordance with the requirements of the State Pharmacopoeia of Ukraine for such characteristics as the range of application, linearity, precision, correctness and robustness, and the predicted total uncertainty of the method was calculated. The range of application of the technique lies within 61–139 %. The calculated parameters testify to the linearity of the technique in the studied range of application. The calculated one-sided confidence interval does not exceed the maximum permissible uncertainty of the analysis, which indicates the accuracy of the method at the level of convergence. The method is correct, because the systematic error does not statistically differ from zero, that is, the true value of the quantity does not go beyond the established confidence interval. The analyzed solutions are stable for at least 60 minutes, since the systematic error introduced by the instability of the analyzed solutions does not exceed a critical value.
Conclusion. As a result of the study, a simple, rapid and environmentally safe spectrophotometric method for the quantitative determination of 1-benzyl-8-morpholinotheobromine in a substance was developed. It is proved that the developed method is valid according to the characteristics such as linearity, range of application, precision, correctness and robustness.
References
Kovalenko VM. COMPENDIUM 2019 – medicinal products. [КОМПЕНДІУМ 2019 – лікарські засоби] K. : Morion 2019. 2480 p. Ukrainian.
Orhan H. Biotransformation of Drugs and other Xenobiotics as Key Modulator of Toxicity. Curr Drug Metab. 2021. 22(8):582–3. https://doi.org/10.2174/138920022208210915160901.
Jackson KD, Argikar UA, Cho S, Crouch RD, Driscoll JP, Heck C et al. Bioactivation and Reactivity Research Advances – 2021 year in review. Drug Metab Rev. 2022;1-83. https://doi.org/10.1080/03602532.2022.2097254.
Gumber D, Yadav D, Yadav R, Kachler S, Klotz KN. Bronchospasmolytic activity and adenosine receptor binding of some newer 1,3‐dipropyl‐8‐phenyl substituted xanthine derivatives. Chem Biol Amp Drug Des. 2020;95(6):600–9. https://doi.org/10.1111/cbdd.13673.
Seirafianpour F, Mozafarpoor S, Fattahi N, Sadeghzadeh‐Bazargan A, Hanifiha M, Goodarzi A. Treatment of COVID ‐19 with pentoxifylline: Could it be a potential adjuvant therapy? 2020;33(4). https://doi.org/10.1111/dth.13733.
Ma QS, Yao Y, Zheng YC, Feng S, Chang J, Yu B et al. Ligand-based design, synthesis and biological evaluation of xanthine derivatives as LSD1/KDM1A inhibitors. Eur J Med Chem. 2019;162:555–67. https://doi.org/10.1016/j.ejmech.2018.11.035.
Dolhikh OP, Romanenko MI, Ivanchenko DH, Aleksandrova KV, Polishchuk NM. Synthesis, physical, chemical and biological properties of 7-ethylxanthinyl-8-aminoalkanoic acids derivatives. Curr Issues Pharm Med. 2019; 1(29):10–14. DOI: 10.14739/2409-2932.2019.1.158921.
Sargazi S, Shahraki S, Shahraki O, Zargari F, Sheervalilou R, Maghsoudi S et al. 8-Alkylmercaptocaffeine derivatives: antioxidant, molecular docking, and in-vitro cytotoxicity studies. Bioorganic Chem 2021;111:104900. https://doi.org/10.1016/j.bioorg.2021.104900.
Ivanchenko DH, Zhuk YM, Vasyuk SO. Quantitative determination of piperidine 7-propil-3-methylxanthine-8-ylthioacetate. Pharm. Rewiev. 2022;(4):20-5. https://doi.org/10.11603/2312-0967.2022.4.13735.
The State Pharmacopoeia of Ukraine. [Державна фармакопея України] Kharkiv: Ukrainian Scientific Pharmacopoeia Center of Quality of Medicinal Pro-ducts. 1 ed. Х; 2001; 556 p. Ed. 1. 2004; 520 p. Ed. 2. Kharkiv: Ukrainian Scientific Pharmacopoeia Centerof Quality of Medicinal Products. 2008; 620 p. Ed 3. 2009; 280 p. Ed. 4. 2011; 540 p. Ukrainian.
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