BEHAVIORAL REACTIONS OF RODENTS AFTER ADMINISTRATION OF THE 1,2,3-TRIAZOLO-1,4-BENZODIAZEPINES DERIVATIVES IN THE OPEN FIELD TEST

Authors

DOI:

https://doi.org/10.11603/2312-0967.2023.4.14297

Keywords:

triazolobenzodiazepines derivatives, “open field” test, spontaneous locomotor activity, grooming, mice

Abstract

The aim of the work. Study of the effect of new 1,2,3-triazolo-1,4-benzodiazepine derivatives on the behavioral reactions of rodents in the “open field” test.

Materials and Methods. Before using 1,2,3-triazolo-1,4-benzodiazepines under the code MA-252, MA-253, MA-254, MA-255 and MA-261 in in vivo experiments, the derivatives were triturated with lactose in a ratio of 1: 1000. Behavioral reactions of rodents were evaluated in the “open field” test, during which the number of crossed squares, rearings, exploratory nose-pokes, acts of defecation, urination and grooming were recorded.

Results and Discussion. The locomotor activity, which is characterized by the number of crossed squares, increased with the introduction of the MA-253 derivative in all investigated doses compared to the index in the control group and was not significantly different from the animals' group receiving gidazepam. The largest number of rearings, which is a manifestation of the exploratory activity of the animal under the test conditions, was observed in the experimental groups that received MA-253 and MA-255 derivatives in a dose of 1 mg/kg and the comparison drug gidazepam. Many of the studied groups observed an increase in the number of nose-pokes, but significant changes were only in the groups that received the MA-253 derivative and gidazepam at a dose of 1 mg/kg. The effect on the emotional component of the behavioral reactions of rodents has also been established.

Conclusions. It was found that the MA-253 derivative in a dose of 1 mg/kg produces the most significant changes, increasing locomotor and orientation-exploratory activity, while reducing the number of emotional manifestations in animals, which in terms of effect is similar to the reference drug gidazepam. Such mice behavior may indicate a reduction in the level of anxiety and psycho-emotional stress of animals, as well as the prospect of further studies of pharmacological activity.

Author Biographies

I. V. Botsula, National Pharmaceutical University of the Ministry of Health of Ukraine

postgraduate student of the Department of Clinical Pharmacology and Clinical Pharmacy

I.V. Kireyev, National Pharmaceutical University of the Ministry of Health of Ukraine

DSc (Medicine), professor, head of Department of Clinical Pharmacology and Clinical Pharmacy

O. M. Koshovyi, National Pharmaceutical University of the Ministry of Health of Ukraine

DSc (Pharmacy), professor of the Department of Pharmacognosy and Nutriciology

M. O. Mazur, State Scientific Institution "Institute for Single Crystals" NAS of Ukraine

Engineer of Department of Organic and Bioorganic Chemistry

V. A. Chebanov, State Scientific Institution "Institute for Single Crystals" NAS of Ukraine

DSc (Chemistry), Professor, Corresponding Member of NAS of Ukraine, First Deputy General Director

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Published

2023-11-30

How to Cite

Botsula, I. V., Kireyev, I., Koshovyi, O. M., Mazur, M. O., & Chebanov, V. A. (2023). BEHAVIORAL REACTIONS OF RODENTS AFTER ADMINISTRATION OF THE 1,2,3-TRIAZOLO-1,4-BENZODIAZEPINES DERIVATIVES IN THE OPEN FIELD TEST. Pharmaceutical Review Farmacevtičnij časopis, (4), 70–77. https://doi.org/10.11603/2312-0967.2023.4.14297

Issue

Section

Pharmacological researches of biologically active substances