BEHAVIORAL REACTIONS OF RODENTS AFTER ADMINISTRATION OF THE 1,2,3-TRIAZOLO-1,4-BENZODIAZEPINES DERIVATIVES IN THE OPEN FIELD TEST
DOI:
https://doi.org/10.11603/2312-0967.2023.4.14297Keywords:
triazolobenzodiazepines derivatives, “open field” test, spontaneous locomotor activity, grooming, miceAbstract
The aim of the work. Study of the effect of new 1,2,3-triazolo-1,4-benzodiazepine derivatives on the behavioral reactions of rodents in the “open field” test.
Materials and Methods. Before using 1,2,3-triazolo-1,4-benzodiazepines under the code MA-252, MA-253, MA-254, MA-255 and MA-261 in in vivo experiments, the derivatives were triturated with lactose in a ratio of 1: 1000. Behavioral reactions of rodents were evaluated in the “open field” test, during which the number of crossed squares, rearings, exploratory nose-pokes, acts of defecation, urination and grooming were recorded.
Results and Discussion. The locomotor activity, which is characterized by the number of crossed squares, increased with the introduction of the MA-253 derivative in all investigated doses compared to the index in the control group and was not significantly different from the animals' group receiving gidazepam. The largest number of rearings, which is a manifestation of the exploratory activity of the animal under the test conditions, was observed in the experimental groups that received MA-253 and MA-255 derivatives in a dose of 1 mg/kg and the comparison drug gidazepam. Many of the studied groups observed an increase in the number of nose-pokes, but significant changes were only in the groups that received the MA-253 derivative and gidazepam at a dose of 1 mg/kg. The effect on the emotional component of the behavioral reactions of rodents has also been established.
Conclusions. It was found that the MA-253 derivative in a dose of 1 mg/kg produces the most significant changes, increasing locomotor and orientation-exploratory activity, while reducing the number of emotional manifestations in animals, which in terms of effect is similar to the reference drug gidazepam. Such mice behavior may indicate a reduction in the level of anxiety and psycho-emotional stress of animals, as well as the prospect of further studies of pharmacological activity.
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