EVALUATION OF THE INFLUENCE OF THICK EXTRACT FROM SHIITAKE MUSHROOMS ON THE DEVELOPMENT OF INFLAMMATION PROCESSES UNDER THE CONDITIONS OF TESTOSTERONE-INDUCED PROSTATIC HYPERPLASIA IN RATS

Authors

DOI:

https://doi.org/10.11603/2312-0967.2023.3.14169

Keywords:

shiitake mushrooms, thick extract, prostatic hyperplasia, prostate-protective effect;, anti-inflammatory effect

Abstract

The aim of the work. To evaluate the effect of thick extract from shiitake mushrooms on the development of inflammatory processes in rats with testosterone-induced prostate hyperplasia.

Materials and Methods. Experimental work was performed on 56 white male rats, divided into seven groups. The model of testosterone-induced hyperplasia of the prostate gland was reproduced by subcutaneous administration of testosterone propionate to animals for 21 days at a fixed dose. A thick extract of shiitake mushrooms was administered intragastrically daily in parallel with testosterone solution for 3 weeks at a dose of 150 mg per kilogram of rat body weight. The reference drug "Prostatofit" was administered intragastrically, diluted in a ratio of 1:10, in a dose of 1 ml per 100 g of animal body weight. The effectiveness of the thick extract from shiitake mushrooms in the review of anti-inflammatory action was studied by the following biochemical indicators: the number of leukocytes, ESR, the content of C-reactive protein, interleukin-6, tumor necrosis factor-α, levels of testosterone and dihydrotestosterone in the blood serum of white rats.

Results and Discussion. Administration of testosterone propionate subcutaneously for 21 days causes the occurrence of inflammatory processes in the body of animals, which is confirmed by a significant increase in the number of leukocytes, ESR, the level of C-reactive protein, interleukin-6, tumor necrosis factor-α, as well as the concentration of testosterone and dihydrotestosterone in the blood serum of the affected rats. It was experimentally proven that a thick extract from shiitake mushrooms at a dose of 150 mg/kg of animal body weight reliably reduced ESR, the number of leukocytes, the level of C-reactive protein, interleukin-6, tumor necrosis factor-α, testosterone and dihydrotestosterone.

Conclusions. Shiitake extract showed a prostate-protective effect, reducing the level of inflammatory markers under the conditions of benign prostatic hyperplasia in rats induced by the administration of testosterone propionate.

Author Biographies

I. I. Herasymets, I. Horbachevsky Ternopil National Medical University

PhD (Pharmacy), Associate Professor, Department of Pharmacology and Clinical Pharmacology

L. S. Fira, I. Horbachevsky Ternopil National Medical University

DІс (Biology), Professor, Head of the Department of Pharmacy of the Institute of Postgraduate Education

I. I. Medvid, I. Horbachevsky Ternopil National Medical University

PhD (Medicine), Associate Professor, Department of Microbiology, Virology and Immunology

References

Brechka NМ. Indicators of the hormonal status of male rats after the use of drugs of natural origin in conditions of benign prostatic hyperplasia. Ukrainian Journal of Medicine, Biology and Sport. 2021;4(5): 325-31. Ukrainian. DOI: https://doi.org/10.26693/jmbs04.05.325

Shchokina KG, Belik GV, Kutsenko TO, Semeniv DV. The effect of thick burdock extracts on development of model hyperplasia of the prostate gland in rats. Clinical pharmacy. 2017;21(3): 41-5. https://doi.org/10.24959/cphj.17.1433. DOI: https://doi.org/10.24959/cphj.17.1433

Onoja RI, Olumuyiwa Shoyinka SV, Omeke JN, Emejuo NT, Ugwoke SN. Protective effects of Chromolaena odorata extract on experimental benign prostatic hyperplasia in rats. Vet Res Forum. 2023;14(2): 59-64. https://doi.org/10.30466/vrf.2021.537224.3219.

Shakir Saleh H, Yahya Kraidi S, Ali Mahdi W. Potential Ameliorative Effect of Artemisia Absinthium Supplement against Sodium Fluoride-Induced Prostatic Toxicity in Rats. Archives of Razi Institute. 2022;77(2): 907-13. https://doi.org/10.22092/ARI.2022.357126.1982.

Karakovska NYe, Shchokina KH, Drohovoz SМ. Study of the antiandrogenic activity of the extracts of leaves and roots of large burdock. Ukrainian biopharmaceutical journal. 2012;4(21): 40-3. Ukrainian.

Karakovska NYe, Shchokina KH, Drohovoz SМ. Study of the effect of thick extracts of large burdock on the course of turpentine prostatitis. Ukrainian biopharmaceutical journal. 2013;2(25): 41-5. Ukrainian.

Balakrishnan B, Liang Q, Fenix K, Tamang B, Hauben E, Ma L, Zlang W. Combining the Anticancer and Immunomodulatory Effects of Astragalus and Shiitake as an Integrated Therapeutic Approach. Nutrients. 2021;13(8): 2564. https://doi.org/10.3390/nu13082564 DOI: https://doi.org/10.3390/nu13082564

Moyano EG, Pilar LM. Flagellate dermatitis secondary to intake shiitake mushrooms. Atencion primaria. 2021;53(1): 102-3. https://doi.org/10.1016/j.aprim.2019.09.008. DOI: https://doi.org/10.1016/j.aprim.2019.09.008

Herasymets II, Fira LS, Medvid II. Experimental substantiation of the safety of thick extract from shiitake mushrooms. Sciences of Europe. 2020; Vol 2, 53. P. 3-6. Ukrainian

Xu Z, Meenu M, Xu B. Effects of UV-C treatment and ultrafine-grinding on the biotransformation of ergosterol to vitamin D2, physiochemical properties, and antioxidant properties of shiitake and Jew's ear. Food chemistry. 2020;309: 125738. https://doi.org/10.1016/j.foodchem.2019.125738 DOI: https://doi.org/10.1016/j.foodchem.2019.125738

Kuroki T, Lee S, Hirohama M, Taku T, Kumakura M, Haruyama T, Nagata K, Kawaguchi A. Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity. Front Microbiol. 2018;9: 1164. DOI: 10.3389/fmicb.2018.01164. DOI: https://doi.org/10.3389/fmicb.2018.01164

Gross, D., Tolba, R. Ethics in Animal-Based Research. Eur Surg Res. 2015;55(1-2): 43-57. DOI: https://doi.org/10.1159/000377721

The European Parliament. European Directive on the protection of animals used for scientific purposes 2010/63/EU. Brussels: The European Parliament; 2010.

Vlizlo VV, Fedoruk RS. Laboratory methods of investigation in biology, stock-breeding and veterinary. Reference book. Spolom, Lviv. 2012: 764. Ukrainian.

Zhou B, Shu B, Yang J, Liu J, Xi T, Xing Y. C-reactive protein, interleukin-6 and the risk of colorectal cancer: a meta-analysis. Cancer Causes Control. 2014;25(10): 1397-405 https://doi.org/10.1007/s10552-014-0445-8. DOI: https://doi.org/10.1007/s10552-014-0445-8

Jannot AS, Agoritsas T, Gayet-Ageron A, Perneger TV. Citation bias favoring statistically significant studies was present in medical research. J Clin Epidemiol. 2013;66(3): 296-301. https://doi.org/10.1016/j.jclinepi.2012.09.015. DOI: https://doi.org/10.1016/j.jclinepi.2012.09.015

Makodrai YI, Klishch IM. Indicators of endogenous intoxication in rats with benign prostatic hyperplasia against the background of the toxic effect of ethanol. Medical and Clinical Chemistry. 2021;23(4): 10-4. https://doi.org/10.11603/mcch.2410-681X.2021.i4.12727. Ukrainian. DOI: https://doi.org/10.11603/mcch.2410-681X.2021.i4.12727

Zaichenko HV, Ravshanov ТB. Prostate-protective effect of suppositories of a combined composition with indole-3-carbinol and meloxicam on a model of sulpiride-induced benign prostatic hyperplasia. Problems of Endocrine Pathology. 2019;1: 95-103. https://doi.org/10.21856/j-PEP.2019.1.12. Ukrainian. DOI: https://doi.org/10.21856/j-PEP.2019.1.12

Herasymets I, Fira L, Mykhalkiv M, Ivanusa I. Activity of the inflammatory processes in rats during experimental carcinogenesis and the influence of dry extract from reishi mushrooms on them. Pharmacology Online. 2021;3: 405-12.

Published

2023-09-30

How to Cite

Herasymets, I. I., Fira, L. S., & Medvid, I. I. (2023). EVALUATION OF THE INFLUENCE OF THICK EXTRACT FROM SHIITAKE MUSHROOMS ON THE DEVELOPMENT OF INFLAMMATION PROCESSES UNDER THE CONDITIONS OF TESTOSTERONE-INDUCED PROSTATIC HYPERPLASIA IN RATS. Pharmaceutical Review Farmacevtičnij časopis, (3), 50–57. https://doi.org/10.11603/2312-0967.2023.3.14169

Issue

Section

Pharmacological researches of biologically active substances