RESEARCH ON STANDARDIZATION OF THE CENTAURIUM ERYTHRAEA HERB DRY EXTRACT
DOI:
https://doi.org/10.11603/2312-0967.2022.1.13052Keywords:
Centaurium Erythraea, herb, dry extract, secoiridoids, flavonoids, chromatography, spectrophotometryAbstract
The aim of the work. Investigation of the identification and quantification possibility of the Centaurium Erythraea herb dry extract.
Materials and Methods. The dry extract obtained by the fractional maceration method of Centaurium Erythraea herb was like research material; extractant – ethanol (70%, v / v), the ratio of raw materials – extractant was 1:10, the extraction rate – 5, the duration of the one extraction – 24 hours; concentration of liquid extract was performed with a rotary evaporator at 50 ° C temperature; drying – at a temperature of 45-50 ° C in an oven; DER - (3,5-4,5): 1. Qualitative studies were performed by thin layer chromatography, quantitative determination of flavonoids - spectrophotometrically.
Results and Discussion. Qualitative studies performed by TLC allowed to detect the secoiridoid glycosides, in particular, swertiamarin was identified. Rutin has been identified among flavonoids. The quantitative content of flavonoids in terms of rutin and dry matter in the test extract was (6.91 ± 0.04)%.
Conclusions. It is proposed to include TLC methods for the secoyridoids and flavonoids identification and spectrophotometric methods for the flavonoids quantitative determination in the draft specification for the Centaurium Erythraea herb dry extract.
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