ANTIMICROBIAL ACTIVITY OF TINCTURES AND LIQUID EXTRACTS FROM THE ROOTS OF ARCTIUM LAPPA, ARCTIUM MINUS AND ARCTIUM TOMENTOSUM
DOI:
https://doi.org/10.11603/2312-0967.2021.4.12512Keywords:
Arctium, root, tincture, liquid extract, antimicrobial activityAbstract
The aim of the work was to determine the antimicrobial activity of tinctures and liquid extracts from the roots of Arctium lappa, Arctium minus and Arctium tomentosum.
Materials and Methods. Tinctures and liquid extracts from plant raw materials were prepared by the method of maceration, extractant 50% ethyl alcohol. The study of antimicrobial activity was performed by the method of diffusion into agar and the method of serial dilutions. The minimum inhibitory and minimum bactericidal concentration of the liquid extracts were determined.
Results and Discussion. Liquid extracts from the root of Arctium lappa, Arctium minus and Arctium tomentosum showed higher antimicrobial activity than tinctures. All strains of microorganisms were most sensitive to the liquid extract of the root of Arctium minus. Tinctures and liquid extracts showed much higher activity against the microorganisms Basillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 25923. The minimum inhibitory and minimum bactericidal concentration of liquid extracts from the root of Arctium lappa and Arctium minus against Escherichia coli ATCC 25922 (for 125 mg/ml and 250 mg/ml, respectively), liquid extract from the root of Arctium minus against Proteus vulgaris ATCC 4636 (250 mg/ml and 500mg/ ml, respectively) and liquid extract from the root of Arctium lappa in relation to Basillus subtilis ATCC 6633 (125 mg/ml and 250 mg/ml, respectively) were at the level of minimum inhibitory and minimum bactericidal concentration of chlorophyllipt in relation to these strains of microorganisms.
Conclusions. To determine the spectrum of antimicrobial activity of tinctures and liquid extracts from the root Arctium lappa, Arctium minus and Arctium tomentosum in relation to 6 museum strains of microorganisms and determine the minimum inhibitory and minimum bactericidal concentration of liquid extracts. Liquid extracts showed higher antimicrobial activity than tinctures. The obtained data will be used in further studies of tinctures and liquid extracts.
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