CHROMATOGRAPHIC ANALYSIS OF ESSENTIAL OILS OBTAINED FROM THE LEMON BEEBALM HERB IN THE DIFFERENT VEGETATION PHASES

Authors

DOI:

https://doi.org/10.11603/2312-0967.2021.1.11936

Keywords:

Monarda citriodora, essential oil, gas chromatography, thin layer chromatography, thymol, carvacrol, chemotype

Abstract

The aim of the work. Chromatographic analysis of essential oils obtained from the lemon beebalm (Monarda citriodora Cerv. ex Lag, Lamiaceae Martinov family) herb in the different phases of vegetation.

Materials and Methods. The herb of M. citriodora was harvested in the experimental plots of Kherson region (Ukraine) during two phases of vegetation: beginning of flowering and total flowering. The essential oil was obtained by hydrodistillation. Its component was studied by gas chromatography with mass spectrometry method (GC/MS). The thin layer chromatography (TLC) was used to obtain the "chromatographic fingerprints" of the essential oil.

Results and Discussion. The component composition of M. citriodora essential oils was studied by GC/MS. There were identified 28 components in the essential oil obtained from the herb during the beginning of flowering, and 27 compounds were found during the total flowering of plants. The predominant components of both essential oils were aromatic monoterpenoids: thymol and carvacrol. Harvesting the M. citriodora raw materials during the total flowering was determined to be more promising because of a higher total content of thymol and carvacrol (84.07 %) in the essential oil compared to the beginning of flowering period (75.09 %). It is proposed to use the TLC analysis of essential oil harvested during mass flowering as an express method for its identification.

Conclusions. The component composition and specific features of the essential oils of the M. citriodora raw materials produced in Kherson region of Ukraine during two different phases of vegetation were revealed. The thymol-carvacrol chemotype of the investigated essential oil was determined. The study of the potential of the pharmacological activity of the investigated essential oil could be considered as very prospective direction of future research.

Author Biographies

M. I. Shanaida, I. Horbachevsky Ternopil National Medical University

PhD (Biology), Associate Professor of the Department of Pharmacognosy and Medical Botany

L. V. Svydenko, Rice Institute of the NAAS, Ukraine

PhD (Biology), Senior Researcher of the Sector of Mobilization and Conservation of Plant Resources

N. V. Hvozdyk, I. Horbachevsky Ternopil National Medical University

fifth-year student of Pharmaceutical Faculty

N. I. Hudz, Danylo Halytsky Lviv National Medical University

PhD (Pharmacy), Associate Professor of the Department of Drug Technology and Biopharmacy

References

Bedi S, Tanuja, Vyas SP. A handbook of aromatic and essential oil plants (cultivation, chemistry, processing and uses). Jodhpur: Agrobios, 2010.

Sharifi-Rad J, Sureda A, Tenore GC, Daglia M, Sharifi-Rad M, Marco Valussi M et al. Biological activities of essential oils: from plant chemoecology to traditional healing systems. Molecules. 2017;22: 70.

Vorobets N, Rivis O. Actuality and perspectives of using medicinal plants for the treatment of oral candidiasis. Visnyk problem biolohii i medytsyny. 2017;1(135): 22-32. Ukrainian.

Shanayda MI, Pokryshko OV. Antimicrobial activity of essential oils of plants belonging to Lamiaceae Juss. family. Annals of Mechnikov Institute. 2014;4: 66-9. Ukrainian.

Karpiński TM. Essential oils of Lamiaceae family plants as antifungals. Biomolecules. 2020;10(1): 103.

Kot B, Wierzchowska K, Piechota M, Czerniewicz P, Chrzanowski G. Antimicrobial activity of five essential oils from Lamiaceae against multidrug-resistant Staphylococcus aureus. Nat. Prod. Res. 2019;33(24): 3587-91.

Lee JH, Kim YG, Lee J. Carvacrol-rich oregano oil and thymol-rich thyme red oil inhibit biofilm formation and the virulence of uropathogenic Escherichia coli. J. Appl. Microbiol. 2017;123(6): 1420-8.

Shanaida M, Hudz N, Korzeniowska K, Wieczorek PP. Antioxidant activity of essential oils obtained from aerial part of some Lamiaceae species International Journal of Green Pharmacy. 2018;12(3): 200-4.

Fraternale D, Giamperi L, Bucchini A, Ricci D, Epifano F, Burini G, Curini M. Chemical composition, antifungal and in vitro antioxidant properties of Monarda didyma L. essential oil. J Ess Oil Res. 2006;18: 581-5.

The State Pharmacopoeia of Ukraine: Ukrainian Scien¬tific Pharmacopoeia Center of Quality of Medicinal Prod¬ucts. Ed.2. [Державна Фармакопея України: ДП «Український науково-експертний фармакопейний центр якості лікарських засобів». 2-е вид. Харків: ДП «Український науково-експертний фармакопейний центр якості лікарських засобів»] Kharkiv: Ukrainian Scientific Pharmacopoeia Center of Quality of Medicinal Products. 2014. Ukrainian.

Vronska LV. pplication of thin layer chromatography method for lemon balm herb identification. Farm chasop. 2011;4: 64-7. Ukrainian.

Vysochina, G.I. Genus Monarda (Lamiaceae): chemical composition, biological activity and practical application (a review). Chemistry in the Interest of Sustainable Development. 2020; 28(2): 105-20. Russian.

Fedotov SV. Essential oils of Monarda fistulosa L., Monarda didyma L., Monarda citriodora Cervantes ex Lag., their chemotypes and biological. Sbornik nauchnych trudov SNBG. 2015; 141. Available from: http://www.real-aroma.ru/period_izdania/sbornik_GNBS/monarda.htm Russian.

Deepika, Singh A, Chaudhar AK, Das S, Dubey NK. Nanoencapsulated Monarda citriodora Cerv. ex Lag. essential oil as potential antifungal and antiaflatoxigenic agent against deterioration of stored functional foods. J Food Sci Technol. 2020;57(8): 2863-76.

Pathania AS, Guru SK, Verma MK, Sharma G, Abdullah ST, Fayaz Malik F et al. Disruption of the PI3K/AKT/mTOR signaling cascade and induction of apoptosis in HL-60 cells by an essential oil from Monarda citriodora. Food and chemical toxicology. 2013;62: 246-54.

Nagoor Meeran MF, Javed H, Al Taee H, Azimullah S, Ojha SK. Pharmacological properties and molecular mechanisms of thymol: prospects for its therapeutic potential and pharmaceutical development. Front Pharmacol. 2017;8: 380.

Hudz N, Makowicz E, Shanaida M, Białoń M, Jasicka-Misiak I, Yezerska O, Svydenko L, Wieczorek PP. Phytochemical evaluation of tinctures and essential oil obtained from Satureja montana herb. Molecules. 2020;25(20): 4763.

Memar MY, Raei P, Alizadeh N, Aghdam MA, Kafil HS. Carvacrol and thymol: strong antimicrobial agents against resistant isolates. Reviews in Medical Microbiology. 2017;28(2): 63-8.

Ghosh M, Schepetkin IA, Özek G, Özek T, Khlebnikov AI, Damron DS, Quinn MT. Essential oils from Monarda fistulosa: chemical composition and activation of transient receptor potential A1 (TRPA1) channels. Molecules. 2020;25(21): 4873.

Li H, Yang T, Li F-Y, Yao Y, Sun Z-M. Antibacterial activity and mechanism of action of Monarda punctata essential oil and its main components against common bacterial pathogens in respiratory tract. Int J Clin Exp Pathol. 2014;7(11); 7389-98.

Mohammadi S, Tabrizi L, Shokrpour M, Hadian J, Schulz H, Riewe D. Morphological and phytochemical screening of some Thymus ecotypes (Thymus spp.) native to Iran in order to select elite genotypes. Journal of Applied Botany and Food Quality. 2020;93: 186-96.

Lu ZG, Li XH, Li W. Chemical composition of antibacterial activity of essential oil from Monarda citriodora flowers. Advanced Materials Research. 2011;183: 920-3.

Di Vito M, Bellardi G, Mondello F, Modesto M, Michelozzi M, Bugli F et al. Monarda citriodora hydrolate vs essential oil comparison in several anti-microbial applications. Industrial Crops and Products. 2019;128: 206-12.

Frezza C, Venditti A, Serafini M, Bianco A. Phytochemistry, chemotaxonomy, ethnopharmacology, and nutraceutics of Lamiaceae. Studies in Natural Products Chemistry. 2019;2: 125-78.

Koshovyi O, Raal A, Kovaleva A, Myha M, Ilina T, Borodina N, Komissarenko A. The phytochemical and chemotaxonomic study of Salvia spp. growing in Ukraine. J Appl Biol Biotech. 2020;8(3): 29-36.

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Published

2021-05-08

How to Cite

Shanaida, M. I., Svydenko, L. V., Hvozdyk, N. V., & Hudz, N. I. (2021). CHROMATOGRAPHIC ANALYSIS OF ESSENTIAL OILS OBTAINED FROM THE LEMON BEEBALM HERB IN THE DIFFERENT VEGETATION PHASES. Pharmaceutical Review Farmacevtičnij časopis, (1), 23–32. https://doi.org/10.11603/2312-0967.2021.1.11936

Issue

No. 1 (2021)

Section

Phytochemical researches

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