RESEARCH OF THE BILBERRY SHOOTS STANDARDIZATION ON THE HYDROXYCINNAMIC ACIDS CONTENT
DOI:
https://doi.org/10.11603/2312-0967.2020.4.11638Keywords:
bilberry shoots, standardization, hydroxycinnamic acids, chlorogenic acid, HPLC, quantitative determinationAbstract
The aim of the work is to quantify of the hydroxycinnamic acids in medicinal plant raw materials of bilberry shoots and substantiate the possibility of it standardization on their content.
Materials and methods. The wild samples of bilberry shoots collected in 4 regions of Ukraine and samples of the drug of bilberry shoots purchased in pharmacies were by material for this study. Electronic absorption spectra and absorption measurements were recorded on a Cary 50 UV-Vis spectrophotometer (Agilent Technologies, USA). The Waters 2690 HPLC-DAD chromatograph (Waters, USA), a XTerra C18 chromatographic column (250x4.6 mm (5 μm), Waters, USA) were used in the study. The chlorogenic, ferulic, rosmarinic and caffeic acids standard samples (Sigma-Aldrich, Fluka) were used for identification and quantification.
Results and discussion. The 15 samples of bilberry shoots: 7 samples of the industrial production drug and 8 samples of the wild raw materials of own colection, have been investigated by HPLC with spectrophometric detection under gradient elution conditions. Has been found, that the chlorogenic acid is the predominant compound of hydroxycinnamic acids and can be used as a standard to recalculate their content. The caffeic and / or ferulic acids have been identified in some test samples and their content is insignificant compared to the chlorogenic acid content. The spectrophotometric determination of the hydroxycinnamic acids amount in the bilberry shoots raw material is impossible due to the interfering effect of flavonoids. The chlorogenic acid content in the bilberry shoots, according to the HPLC determination results was in the range 0.385-0.566% for the drug samples, and in the range 1.368-1.991% for the wild raw materials samples. The chlorogenic acid high content in the bilberry shoots raw material and its biological activity are an argument for the introduction of a new selective quantitative quality indicator for its standardization.
Conclusion. The chlorogenic acid determination by HPLC can be introduced as a quantitative indicator of the bilberry shoots raw material quality to replace the current non-selective indicator - the polyphenols determination.
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