IDENTIFICATION OF THE STRUCTURE OF THE PRODUCT OF VERAPAMIL HYDROCHLORIDE WITH BROMCRESOL GREEN INTERACTION
DOI:
https://doi.org/10.11603/2312-0967.2020.1.10914Keywords:
verapamil hydrochloride, bromocresol green, ionic associate, spectrophotometryAbstract
The aim of the work. Isolation and identification of the product of interaction of verapamil hydrochloride with bromocresol green by IR spectrophotometry and 1H NMR spectroscopy methods.
Materials and Methods. Verapamil hydrochloride working standard, bromocresol green and the sample of final dosage forms of Ukrainian and foreign production were used.
Reagents and solvents: A standard sample of verapamil hydrochloride, bromocresol green, acetone.
Analytical equipments: Spectrophotometer Specord Bruker Alpha (Bruker Optik GmbH, Ettlingen, Germany) using a prefix (full internal reflection) ATR (direct injection of substance), Spectrophotometer Specord 200(Germany) (190-1100 nm).
Results and Discussion. It was experimentally established that verapamil hydrochloride forms coloured ion-pair product with bromocresol green in acetone medium. IR-spectrum ion-pair complex corresponds to the sum of absorption of starting compounds with some differences, which confirm its formation. In ion-pair product in the region 3600‒3400 cm‒1 strips of phenolic –ОН appear with less intensity, which can be explained by the transformation of bromocresol green into the quinone structure. The product also has no absorption in the region 2370cm‒1(R3N+Cl- in verapamil hydrochloride), which is explained by the formation of the ionic associate with bromocresol green, namely, the absorption is manifested in the form of a wider signal in the region 3200‒2000cm‒1.
Conclusions. Presented analytical assay showed that verapamil hydrochloride reacts with bromocresol green in a ratio of 1:1, it was also established and identified the structure of the product of reaction. Using IR- spectroscopy and nuclear magnetic resonance spectrometry, it was confirmed that is known to be an ionic pair.
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