THE PERSPECTIVE OF APPLYING OF DENSE SPINACH LEAVES (Spinacia oleracea L.) EXTRACT AS MEMBRANE PROTECTOR IN CONDITIONS OF TOXIC HEPATITIS
DOI:
https://doi.org/10.11603/2312-0967.2019.3.10407Keywords:
white rats, tetrachloromethane, thick leafy spinach extract, silymarin, membrane-protecting propertiesAbstract
The aim of the work. Investigation of the membrane-protective properties of the dense spinach leaves extract in conditions of toxic liver damage with tetrachloromethane.
Materials and Methods. The experiments were performed on white male rats, which were simulated toxic hepatitis by liver damage with tetrachloromethane. The toxicant was administered at a dose of 1.0 ml/kg body weight of animals. Dense spinach leaves extract was used at a dose of 150 mg/kg body weight, a drug comparison silymarin was used at a dose of 100 mg/kg body weight. Euthanasia of rats was performed under thiopental anesthesia on the 4th, 7th and 10th day of the experiment in compliance with all the rules of the Vertebrate Convention. The activity of membrane-destructive processes was evaluated by alanine and aspartate aminotransferase, gamma-glutamyltranspeptidase activity and erythrocyte intoxication index. Parametric (Student's) and nonparametric (Wilcoxon) methods were used for statistical data processing.
Results and Discussion. It was shown that the destruction of rats with tetrachloromethane leads to a significant increase in serum activity of aminotransferases and gamaglutamyltranspeptidase in all study periods (4, 7 and 10 days from the beginning of the experiment). In the liver of rats, there was a progressive decrease in the activity of enzymes, which is a consequence of impaired permeability of plasma membranes of hepatocytes. In the blood of rats infected with tetrachloromethane, an increase in the percentage of penetration of erythrocyte membranes was noted. Used of dense spinach leaves extract had an effective effect on the studied parameters and slightly inferior to the drug silymarin.
Conclusions. The positive effect of the dense spinach leaves extract on the permeability indices of the plasma membranes of hepatocytes and erythrocytes, which were significantly decreased in serum after administration of the extract, was found in an experiment on rats with tetrachloromethane damage of the liver. This confirms the membrane-protective properties of this remedy, which makes it promising to further study of its pharmacological activity.
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