FLAVONOIDS AND HYDROXYCINNAMIC ACIDS CHROMATOGRAPHIC PROFILES OF DOMESTIC RAW MATERIALS SAMPLES OF MULBERRY WHITE LEAVES
DOI:
https://doi.org/10.11603/2312-0967.2019.2.10269Keywords:
mulberry white leaves, raw materials, flavonoids, hydroxycinnamic acids, chromatographic profile, chromatographic fingerprintingAbstract
The aim of the work. Study of flavonoids and hydroxycinnamic acids TLC and HPLC chromatographic profiles of domestic raw material samples of mulberry white leaves.
Materials and methods. Samples of mulberry white leaves raw material were collected in June 2015-2018 in the Ternopil, Volyn, Kharkiv and Mykolaiv regions, two samples were purchased in Poland in different years – a nutritional supplement "Morwa biała" (crushed leaves, APTEO NATURA, Poland).
Silica gel 60 F254 chromatographic plates (Merck, Germany), Linomat 5 (CAMAG, Switzerland) – device for drawing samples were used in TLC studies. In HPLC studies of flavonoids and hydroxycinnamic acids, the chromatographs Agilent 1200 with G1315D DAD Detector (“Agilent”, USA) and Waters Alliance System 2690 with Waters 996 DAD Detector (“Waters”, USA), the chromatographic column XTerra C18 ("Waters", USA) and gradient elution were used.
Results and discussion. As a result of chromatographic studies of domestic samples of mulberry white leaves, collected in different regions of Ukraine, it has been established, that rutin, isoquercitrin, kaempferol-3-O-β-D-glucoside and chlorogenic acid are the main among of flavonoids and hydroxycinnamic acids. Quercitrin and ferulic acid are identified but their content is low, which is consistent with the literature data on the phytochemical analysis of Polish, Serbian, Chinese, Korean, and Hungarian samples of raw material. In TLC-profiles, in addition to the above-mentioned biologically active substances, there are characteristic intensive zones that are likely to belong to other flavonoids and hydroxycinnamic acids. Similarly, HPLC profiles also contain peaks of unidentified substances whose absorption spectra are common for flavonoids and hydroxycinnamic acids.
Conclusions. The obtained TLC and HPLC chromatographic profiles of flavonoids and hydroxycinnamic acids are similar for various samples and can be used to develop methods for identifying of mulberry white leaves plant material and dugs with it. Rutin, isoquercitrin, kaempferol-3-O-β-D-glucoside and chlorogenic acid should be selected as active markers for the subsequent development of methods for identification this plant material using the "chromatographic fingerprinting" method.
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