THE EFFICIENCY OF PROBIOTIC BACTERIA IN COMBINED VACCINATION AGAINST HEPATITIS B IN EXPERIMENTAL STUDIES

Authors

  • L. M. Lazarenko D. Zabolotnyi Institute of Microbiology and Virology of NAS of Ukraine; PJSC «SPC Diaproph-Med», Kyiv
  • L. M. Hanova D. Zabolotnyi Institute of Microbiology and Virology of NAS of Ukraine; PJSC «SPC Diaproph-Med», Kyiv
  • L. P. Babenko D. Zabolotnyi Institute of Microbiology and Virology of NAS of Ukraine; PJSC «SPC Diaproph-Med», Kyiv
  • O. K. Kyseliova D. Zabolotnyi Institute of Microbiology and Virology of NAS of Ukraine; PJSC «SPC Diaproph-Med», Kyiv
  • V. O. Shevchuk D. Zabolotnyi Institute of Microbiology and Virology of NAS of Ukraine; PJSC «SPC Diaproph-Med», Kyiv
  • M. Ya. Spivak D. Zabolotnyi Institute of Microbiology and Virology of NAS of Ukraine; PJSC «SPC Diaproph-Med», Kyiv

DOI:

https://doi.org/10.11603/1681-2727.2017.4.8422

Keywords:

hepatitis B, vaccination, lactobacilli, bifidobacteria, enzyme-linked immunosorbent assay.

Abstract

The aim of the work – to elucidate the efficacy of three times immunizations of BALB/c mice with recombinant Engerix-B vaccine, as well as its combinations with lactobacilli and bifidobacteria probiotic strains for hepatitis B prevention.

Materials and methods. The recombinant Engerix-B (ENGERIX B) vaccine from GlaxoSmithKline Biologicals S.A. (Belgium) was used in the work. that administered to mice subcutaneously along the spine once a day, three times with an interval of 10 days. The formation of antibo­dies to the HBsAg subtypes ad and ay in serum blood was studied by enzyme-linked immunosorbent assay.

Conclusions. The use of Engerix-B vaccine in conjunction with L. casei IMV B-7280 increases the effectiveness of hepatitis B vaccination in animals, as evidenced by a significant increase in antibody titres after the third immunization.

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Published

2017-12-29

How to Cite

Lazarenko, L. M., Hanova, L. M., Babenko, L. P., Kyseliova, O. K., Shevchuk, V. O., & Spivak, M. Y. (2017). THE EFFICIENCY OF PROBIOTIC BACTERIA IN COMBINED VACCINATION AGAINST HEPATITIS B IN EXPERIMENTAL STUDIES. Infectious Diseases – Infektsiyni Khvoroby, (4), 43–48. https://doi.org/10.11603/1681-2727.2017.4.8422

Issue

No. 4 (2017)

Section

Original investigations

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