ANTIBIOTIC SUSCEPTIBILITY OF BIOFILM PRODUCING MICROORGANISMS ISOLATED FROM GUNSHOT WOUNDS

Authors

  • V. P. Kondratiuk M.I. Pyrohov Vinnytsia National Medical University
  • V. P. Kovalchuk Military Medical Clinical Centre Central Region
  • I. M. Kovalenko Military Medical Clinical Centre Central Region

DOI:

https://doi.org/10.11603/1681-2727.2016.4.7214

Keywords:

bacterial biofilm, antibiotic resistance, antibiotic concentration.

Abstract

Currently it is unclear whether direct correlation between biofilm formation and antibiotic resistance exist. In this study, we examined the relationship between antibiotic resistance and biofilm formation in clinical isolates.

Thirteen isolates were collected from gunshot wounds during 2014. Biofilm-forming capacities were evaluated using the crystal violet staining method. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum biofilm eradication concentration (MBEC) to amikacin, ceftriaxone, сefaperazon/clavulanic acid, ciprofloxacin, meronem, gatifloxacin, and levofloxacin were evaluated using micro dilution assays.

Susceptible in planktonic strains showed high resistance in form of biofilm. The MBEC of tested antibiotics for biofilmforming strains exeeded 500mcg/ml and was more than a hundred times higher than clinically attainable concentration. However, the correlation between the studied properties of A. baumannii strains was diverse: for meronem (r = –0,62), for cefoperazone with clavulanic acid (r = +0,62), for amikacin (r = +0,84). P. aeruginosa strains showed contradictory relation between biofilm forming and resistance. Results from this study imply that biofilm formation and antibiotic resistance acts as a separate mechanism for bacteria to get better survival, especially in isolates with weak resistance level.

Biofilm formation and antibiotic resistance acts as a separate mechanism for bacteria to get better survival, especially in isolates with weak resistance level.

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Published

2017-02-10

How to Cite

Kondratiuk, V. P., Kovalchuk, V. P., & Kovalenko, I. M. (2017). ANTIBIOTIC SUSCEPTIBILITY OF BIOFILM PRODUCING MICROORGANISMS ISOLATED FROM GUNSHOT WOUNDS. Infectious Diseases – Infektsiyni Khvoroby, (4), 52–57. https://doi.org/10.11603/1681-2727.2016.4.7214

Issue

No. 4 (2016)

Section

Original investigations

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