EXPERIMENTAL STUDY OF P. AERUGINOSA AUTOVACCINES RECEIVED BY A MODIFIED PHOTODYNAMIC METHOD

Abstract

SUMMARY. The purpose of this research is usage to improve the effectiveness of treatment of Pseudomonas aeruginosa infection by stagnation of autovaccines, withdrawn from and the method of photodynamic inactivation of the alarm system.

Materials and methods. Experimental series of autovaccines were prepared using a modified photodynamic method. For further investigations, 150 white outbred mice were used, weighing 18–20 g. For infecting laboratory animals, an LD50 strains of R a was prescribed. The effectiveness of vaccine therapy was determined by equal rates of target survival in the last and control groups.

Results. The results are being followed. The experiment showed the prospects of stagnation of autovaccines when previously infected with a homologous strain of P. The results obtained indicate the high effectiveness of vaccine therapy for infected animals, based on the survival rate in up to the last group accumulated 71.8 %, which was 3 times higher than that of the control group. Vaccination during the period of clinical manifestations of infection (3–5 days after infection) was also effective, but the number of dead animals was significantly smaller (p<0.05). Moreover, in the absence of regular clothing, treatment with autovaccine significantly prolonged their lives (up to 20–30 days) and changed the stage of manifestation of clinical symptoms of illness.

Conclusion. Experimental studies have shown that the use of isolated methods for synonymous autovaccines can provide high protective and therapeutic activity (three times the number of surviving animals), so it is better to study the specific characteristics of the autovaccines. accinoterapies are relevant and promising.