BACTERIOPHAGES: CURRENT ISSUES OF PHASE PREPARATION AND EVALUATION OF THEIR ACTIVITY

Abstract

The problematic issues of the doctrine of bacteriophages, their features, methods of detecting phage sensitivity, prospects for the use of phage drugs for therapeutic purposes are highlighted.

The effectiveness of the lytic action of phages depends not only on their specificity and virulence, but also on a number of conditions of use. However, along with the declared effectiveness of phages, their practical application does not always achieve the desired effect. Thus, it is possible that in the process of sequential reception for 7–10 days is the adaptation of the phage and the selection of the most active phage parts.

Not only hospital, but also outpatient, spontaneously changing strains of opportunistic pathogens (UPM) circulate in each region. Accordingly, a significant number of bacteriophage strains circulate in different cells, most of which have low virulence.

True lysogenicity is registered, as well as pseudolysogeny. In this case, in contrast to truly lysogenic strains, pseudolysogenic are a mixture of phages and cultures that are resistant to a particular bacteriophage. In this case, part of the sensitive cells can be lysed, and the rest remains resistant, but does not contain prophage.

Knowledge of the features of bacteriophages avoids a number of errors both in obtaining diagnostic and therapeutic drugs-phages, and in their use for practical purposes, ie to achieve high efficiency of phage therapy.