THE RECOVERY OF VAGINAL MICROBIOM IN BACTERIAL VAGINOSIS BY DIALAC PROBIOTICS

Authors

  • H. I. Mykhaylyshyn I. Horbachevsky Ternopil National Medical University
  • S. І. Klуmnyuk I. Horbachevsky Ternopil National Medical University
  • M. Y. Spivak D.K. Zabolotny Institute of Microbiology and Virology NAS of Ukraine
  • L. M. Lazarenko D.K. Zabolotny Institute of Microbiology and Virology NAS of Ukraine
  • L. M. Malanchuk I. Horbachevsky Ternopil National Medical University
  • I. V. Korda I. Horbachevsky Ternopil National Medical University

DOI:

https://doi.org/10.11603/1681-2727.2020.4.11460

Keywords:

bacterial vaginosis, vaginal microbiome, probiotic, lactobacilli, aerobic and anaerobic pathogens, commensals

Abstract

The article presents the results of study of the vagina microbiome in women with bacterial vaginosis before and after oral use of the capsule form of the probiotic “Dialak” containing strain L. casei IMB B-7280 and vaginal suppositories with the same probiotic. This drug is recommended for the correction of vaginal microbiota, and it has combined anti-inflammatory and selective antimicrobial activity. It has a high antagonistic activity against pathogenic and opportunistic microorganisms: Staphylococcus spp, Streptococcus spp, Pseudomonads spp., Escherichia coli, etc. It effectively inhibits the growth of microscopic fungi.

The aim of the study – to evaluate the effectiveness of the probiotic “Dialak” for women with bacterial vaginosis.

Patients and methods. The examination of women with bacterial vaginosis was performed by traditional gynecological examination to determine the general history of disease particularly, to determine the frequency, severity of clinical manifestations, and duration of recurrence of urogenital infection. Laboratory diagnostic methods included microscopy of the smears for evaluation microbial status for confirmation of the presense of key cells, as well aminotest, examination of secrets pH, classical bacteriological technique before treatment in 10 days after treatment, examination of peripheral blood before treatment and in a month after probiotic therapy to study cellular immunity.

Results. Examination showed that due to vaginal and oral use of probiotic “Dialak” there is stable colonization of vaginal mucosa with commensal microbiota. The effectiveness of the drug against Gardnerella vaginalis, Mobiluncus spp, Lactococcus spp, Veillonella spp is clinically proven.

Conclusion. The possibility of using the probiotic “Dialak” containing strain L. casei IMB B-7280 for the treatment of bacterial vaginosis has been clinically proven. Good tolerability of the medicine, absence of side effects, convenient form of use, justify its use both in obstetrics and gynecology for the treatment of bacterial vaginosis and correction of vaginal micropbiota.

Author Biographies

H. I. Mykhaylyshyn, I. Horbachevsky Ternopil National Medical University

Assistant of the Department of Microbiology, Virology and Immunology, I. Horbachevsky Ternopil National Medical University, Ministry of Health of Ukraine

S. І. Klуmnyuk, I. Horbachevsky Ternopil National Medical University

MD, Professor, Head of the Department of Microbiology, Virology and Immunology, I. Horbachevsky Ternopil National Medical University, Ministry of Health of Ukraine

M. Y. Spivak, D.K. Zabolotny Institute of Microbiology and Virology NAS of Ukraine

Corresponding Member of the National Academy of Sciences of Ukraine, PhD (Biological Sciences), Professor, Head of the Department of Interferon and Immunomodulators, D. K. Zabolotny Institute of Microbiology and Virology

L. M. Lazarenko, D.K. Zabolotny Institute of Microbiology and Virology NAS of Ukraine

Corresponding Member of the National Academy of Sciences of Ukraine, PhD (Biological Sciences), Professor, Head of the Department of Interferon and Immunomodulators, D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine

L. M. Malanchuk, I. Horbachevsky Ternopil National Medical University

MD, Professor, Head of the Department of Obstetrics and Gynecology No.1, I. Horbachevsky Ternopil National Medical University, Ministry of Health of Ukraine

I. V. Korda, I. Horbachevsky Ternopil National Medical University

Doctor of Medicine, Associate Professor of Obstetrics and Gynecology No. 2, I. Horbachevsky Ternopil National Medical University, Ministry of Health of Ukraine

References

Nikitina, I.M. (2017). Peculiarities of Biocenosis and functional activity of vaginal epithelium in local treatment of nonspecific vaginitis. Aktualni pytannia pediatrii, akusherstva ta hinekolohii – Actual Issues of Pediatrics, Obstetrics and Gynecology, 2, 61-66 [in Ukrainian].

Kira, Ye.F., & Muslimova, S.Z. (2008). Non-specific vaginitis and its effect on the reproductive health of women. Problemy reproduktsii – Problems of Reproduction, 5, 8-14 [in Russian].

Klymnyuk, S.I., Mukhailyshyn, H.I., & Malanchuk, L.M. (2019). Microbiological features of bacterial vaginosis in women of different ages and ways of their microbiological correction. Zdobutky klinichnoi i eksperymentalnoi medytsyny – Proceedings of Clinical and Experimental Medicine, 3, 22 [in Ukrainian].

Dyudyun, A.D., & Kruzhnova, O.V. (2013). Complex treatment of patients with bacterial vaginosis taking into account microbiotic and immune disorders. Dermatovenerolohiia. Kosmetolohiia.

Seksopatolohiia. – Dermatovenereology. Cosmetology. Sexual Pathology, 1-4, 21-29 [in Ukrainian].

Vdovychenko, Yu.P., & Hopchuk, O.M. (2016). Bacterial vaginosis – monotherapy with combined drugs. Zdorovye zhenshchiny – Health of Women, 1(107), 132-136 [in Ukrainian].

Ananyeva, M.M. (2018). Etiological and patogenetic aspects of non-specific bacterial vagiosis. Zaporozhskyy medytsynskyy zhurnal – Zaporozhye Medical Journal, 20, 3 (108) [in Ukrainian].

Tsmur, O.V., Levchuk, O.B., Lyashyna, K.V., & Boyko, N.V. (2016). The results of the use of the domestic synbiotic Bifiten for the treatment of bacterial vaginosis in pregnant women. Zdorovye zhenshchiny – Health of Women, 6 (112), 66-72 [in Ukrainian].

Kaminskyy, V.V., Konoplyanko, V.V., Vorobey, L.I., & Tkachuk, R.R. (2014). Efficacy of oral probiotics to restore the vaginal flora. Zdorovye zhenshchiny – Health of Women, 6 (92) [in Ukrainian].

Hopchuk, O.M., & Morozova, O.V. (2015). Strategies for influencing the vaginal biocenosis in women at risk. Zdorovye zhenshchiny – Health of Women, 6, 81-83 [in Ukrainian].

Downloads

Published

2021-03-19

How to Cite

Mykhaylyshyn, H. I., Klуmnyuk S. І., Spivak, M. Y., Lazarenko, L. M., Malanchuk, L. M., & Korda, I. V. (2021). THE RECOVERY OF VAGINAL MICROBIOM IN BACTERIAL VAGINOSIS BY DIALAC PROBIOTICS. Infectious Diseases – Infektsiyni Khvoroby, (4), 18–23. https://doi.org/10.11603/1681-2727.2020.4.11460

Issue

No. 4 (2020)

Section

Original investigations

License

Copyright (c) 2021 Infectious Diseases

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Journal Infectious Disease (Infektsiini Khvoroby) allows the author(s) to hold the copyright without registration

Users can use, reuse and build upon the material published in the journal but only for non-commercial purposes

 Лицензия Creative Commons

This journal is available through Creative Commons (CC) License  BY-NC "Attribution-NonCommercial" 4.0