ANALYSIS OF IMMUNOBLOTTING DIAGNOSTIC PARAMETERS FOR DETERMINATION OF SENSIBILIZATION TO POLLEN ALLERGENS IN PATIENTS WITH RESPIRATORY DISEASES
DOI:
https://doi.org/10.11603/1681-2727.2020.1.11096Keywords:
prick-test, allergy, immunoblotting, IgEAbstract
The aim of the study was to compare the diagnostic parameters of different systems of serological examination by immunoblot method for the determination of sensitization to allergens of pollen group in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma.
Materials and methods. In the study, 88 patients with allergic rhinitis and/or atopic asthma were examined with three different methods of specific allergic diagnosis (in vivo and in vitro). Inclusion criteria were a diagnosis of allergic rhinitis (both intermittent and persistent) and/or atopic asthma. The pre-test was conducted according to the classic test method according to the normative documents with commercial allergen extracts. Western blots for the determination of IgE levels were performed using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun).
Results and conclusions. The results of two systems for determining specific IgE for pollen allergens by the Rida AllergyScreen and Euroline methods do not always agree very well due to the systematic divergence of indicators.
The results of determining specific IgE for individual allergens are generally in good agreement with each other and the results of skin testing by the prick test, however, for pollen, birch, hazel and alder pollen allergens, the Euroline system does not always match the results of skin testing by the prick test. negative results. Further analysis of the consistency and diagnostic parameters of the methods for other allergen groups is necessary to summarize all the results of the study.
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